Tuesday, 11 September 2018

"The quality of the results obtained with this product are sub-optimal. Not so great!"

PRODUCT:                 DRAQ5

Application Area:       Analyzed nuclei in Drosophila ovaries using Draq5


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐
 Average rating:          4.0


Comments: The product itself is easy to use but the results are not reproducible every time.  Would love if the product quality is improved because overall it is a good reagent to have!

Name: 
Tanu Singh

Organisation: Fox Chase Cancer Center

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Manufacturer's response: The initial review posting elicited an immediate technical support response. After successful testing of both fresh DRAQ5 and DRAQfx (for comparison) in the customer’s lab the issue was isolated to the presence of 0.3% Triton X-100 during the final counterstaining step. This was remedied by an intermediate wash with PBS. This learning will be reflected in our products’ technical data sheets for fixed cell immunofluorescence protocols.

Friday, 7 September 2018

"Good product"

PRODUCT:                 DRAQ5

Application Area:       Highlight nuclei in mitosis imaging


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐
Average rating:           4.3


Comments: The product is very easy to use and I followed the manufacturer's instructions. It works very well and does staind the nuclei. Unfortunately 6 months after opening the quality got lower since precipitates were shown in my images. I kept it at 4° as requested. It would be useful to be able to buy smaller aliquots for labs only using it to stained fixed cells.

Name: 
Emanuela Pupo

Organisation: Candiolo Cancer Institute

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Manufacturer’s response: The presence of precipitates in the vial is most unusual and could only be a result of contamination or freezing (accidental, most usually). We would like to have explored this with you at the time you saw this. Your comment about smaller quantities for fixed cell staining is well made - we have developed a product exactly of this nature - please see DRAQfx which is specifically for fixed cells, provided in a convenient dropper bottle. <A replacement vial of DRAQ5 was provided free-of-charge>.