Thursday, 24 March 2016

"Outstanding results, isn't the cheapest nuclear label, but works well"

PRODUCT:                 DRAQ5

Application Area:       Live cell imaging


Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐
Value for Money:        ⭐⭐⭐
 Average rating:          4.3

Comments: DRAQ5 works well as a nuclear label and allows us to use a different spectral range for configuring our multi-label assays.  It appears (not 100% sure why) that DRAQ5 has less particulate and non-specific labelling in our microvascular endothelial cells than Hoechst. 

Name: 
Silas Leavesley

Organisation: University of South Alabama

"Draq5 is an easy and efficient fluorescent Live-Cell Permeant DNA Dye to perform accurate normalization in Cell Western Blotting."

PRODUCT:                 DRAQ5

Application Area:       Cell number normalization of fluorescence signal between wells in cell Western Blotting


Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐
Value for Money:        ⭐⭐⭐
 Average rating:          4.3

Comments: The product Draq5 is a ready-to-use aqueous formulation really easy to use (simple incubation and washes) and it gives us a good reproducibility between experiment. 
It has a good stability after storage.
Draq5 is a stable fluorescent Live-Cell Permeant DNA Dye which allow us to normalize the fluorescence signal between wells in an in Cell Western Blotting (Odyssey). We followed protein expression (Fluorescence 1) in bone marrow derived macrophages after different cell culture conditions and Draq5 signals (Fluorescence 2) provided us a good linearity for normalization of protein signals well-to-well (ration Florescence1/Fluorescence2).

Name: 
François CANONNE-HERGAUX

Organisation: INSERM , Intitut de Recherche en Santé Digestive (IRSD), Toulouse, France