"excellent product to safely immobilise motile cells"

PRODUCT:                 CyGEL
Application Area:        Live cell fluorescent imaging of Leishmania promastigotes

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments:                 Without Cygel it would be almost impossible to obtain high-quality microscopy of motile forms of Leishmania parasites. The product is easy to use and gives a reproducible performance. I've used it to image Leishmania promastigotes that were modified to contain fluorescent proteins, in order to gain information on protein function. Cellular morphology remained unchanged by the embedding process and protein localization was not affected. Can highly recommend Cygel.

Name: Nathaniel Jones

Organisation: University of York

"excellent results"

PRODUCT:                 CyGEL
Application Area:        Leishmania mexicana live cell imaging

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments:                 CyGel was very convenient to use and I would recommend it to everyone working on leishmania.

Name: Jaspreeet Grewal

Organisation: University of Warwick

"excellent for mounting live cells for microscopy"

PRODUCT:                 CyGEL
Application Area:        Mounting bacteria for live cell confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 Cygel has been a great addition to my microscopy toolbox! It enables me to carry out imaging of motile bacteria without needing to fix them. Very easy to use, and seems to go a long way. Can highly recommend.

Name: Rhia Stone

Organisation: Institute for Molecular Bioscience, University of Queensland

"excellent product, affordable, easy to use and gave great results"

PRODUCT:                 CyGEL
Application Area:        Imaging non-adherent leukemia cell line for biosensor application.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 We used CyGel for imaging a K562 cell line to verify that our fluorophore-tagged biosensor is internalized by the cells. The product was very easy to use and worked according to the manufacturer’s description. There was no problem with CyGel interfering with our fluorescent signal using a standard fluorescent microscope for cell imaging. We think it was a very reasonable price for the ease of use and results we obtained. While we did not require follow up service, it was there for our assistance as needed.

Name: Steven Ouellette

Organisation: KinaSense LLC

"I achieved reproducible results in imaging and rescuing zebrafish larva using CyGel"

PRODUCT:                 CyGEL
Application Area:        Live Imaging of Zebrafish Larva

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 Imaging mitochondrial dynamics requires that my sample be completely stationary and alive! CyGel allowed me to stabilize zebrafish larva and obtain time-lapse images of mitochondria. I was able to rescue the fish afterwards, so that fish could be imaged on multiple days if necessary.

Name: April Dukes

Organisation: University of Pittsburgh

"useful product"

PRODUCT:                 CyGEL
Application Area:        Frog embryo research

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments:                 This is an effective product that we find is relatively easy to use.

Name: Tim Mohun

Organisation: The Francis Crick Institute

"reliable, user-friendly product"

PRODUCT:                 CyGEL
Application Area:        Live cell imaging by confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 We have used CyGEL to immobilize Drosophila pupae for live imaging using a confocal microscope. Embedding of samples in glass bottom dishes is carried out using a fluorescence stereomicroscope. We find CyGEL more convenient than LMP agarose because it saves the hassle to melt agarose and have a heating block or water bath nearby to keep agarose liquefied. The only utensil required is a small container with ice to keep CyGEL in its liquid form. Toxicity was not an issue since samples survived 3D time-lapse imaging for five days (see http://rsos.royalsocietypublishing.org/content/3/2/150517). Overall, we are very satisfied with this product.

Name: Martin Wasser

Organisation: BioImagingMW

"wonderful product to immobilize cells"

PRODUCT:                 CyGEL
Application Area:        Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 This is wonderful product to immobilize cells. It can be used also for immobilization of cells after cell sorting. Just sort cells on cool microscope slides and your cells will be immobilized during transport to confocal microscopy (as the slides will heat to room temperature). Just a very easy-to-use tool.

Name: Marek Sinkora

Organisation: Institute of Microbiology of the Academy of Sciences of the Czech Republic