Application Area: Short-term membrane staining
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Average rating: 5.0
Comments: DRAQ9 was a gift from BioStatus. We used it to determine if the vacuoles that we were observing in mammalian cells under hypotonic stress are surrounded by a lipid membrane. DiI that we tried before failed to stain the vacuoles, but with DRAQ9 the result was unequivocally positive: a bright staining was easily detectable under a confocal microscope. The presence of lipid was confirmed with Nile Red. We published the image in Fig. 5 of https://www.nature.com/articles/s41598-024-79815-z.pdf
Name: Michael Model
Average rating: 5.0
Comments: DRAQ9 was a gift from BioStatus. We used it to determine if the vacuoles that we were observing in mammalian cells under hypotonic stress are surrounded by a lipid membrane. DiI that we tried before failed to stain the vacuoles, but with DRAQ9 the result was unequivocally positive: a bright staining was easily detectable under a confocal microscope. The presence of lipid was confirmed with Nile Red. We published the image in Fig. 5 of https://www.nature.com/articles/s41598-024-79815-z.pdf
Name: Michael Model
Organisation: Kent State University