"Nuclei FACs sorting"

PRODUCT:                  DRAQ5

Application Area:        Cell Sorting

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    Not required

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:

Name: Shirin Schneeberger

Organisation: Charite, Germany

Nuclei of mouse spleen cells were stained with Draq5 dye (1:500) (Abcam, Cambridge, UK) and slides were mounted into ProLong ®Gold Antifade Reagent (Cell Signaling Technology, MA, USA).

PRODUCT:                  DRAQ5

Application Area:        Immunofluorescence microscopy

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: To our experience, the best staining was achieved when dye Draq5 was added to cells together with the fluorescent secondary antibody  and incubated at least 1 hr at room temperature.

Name: Gabriela Hrčková

Organisation: Anon., Slovakia

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Manufacturer's response: Dear Gabriela, thank you for your technical feedback on the use of DRAQ5 in immunofluorescence methods - combining antibodies and DRAQ5 is a nice way to streamline a procedure. 

"DRAQ5 for confocal imaging"

PRODUCT:                  DRAQ5

Application Area:        Confocal Imaging

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 was used for nucleus staining in our study. 

Performance: Great 

Easy to handle

Name: Savita Choudhary

Organisation: BITS Pilani Hyderabad Campus

"A Convenient Staining Solution for Phenotypic Screening"

PRODUCT:                  CyTRAK Orange

Application Area:        High Content / Phenotypic Screening

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: We used CyTRAK Orange to stain cultured cells in a phenotypic screening of small-molecule compounds. This dye enabled clear and simultaneous distinction between the nucleus and cytoplasm with a single staining step, consistently delivering reliable results. In our imaging-based phenotypic analysis, CyTRAK Orange significantly simplified image processing compared to dyes that stain only the nucleus, as it provided precise spatial information on both the cytoplasm and nucleus within fluorescent images. Overall, CyTRAK Orange proved to be a convenient and dependable staining solution, making it an invaluable tool in our research.

Name: Junichi Sawada

Organisation: University of Shizuoka, Japan

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Manufacturer's response: Dear Junichi -san, Thank you for this generous review of CyTRAK Orange.  You have cleverly maximised the information that can be extracted from your cell-based assay and its seems CyTRAK Orange played a useful part in that!