"A Convenient Staining Solution for Phenotypic Screening"

PRODUCT:                  CyTRAK Orange

Application Area:        High Content / Phenotypic Screening

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: We used CyTRAK Orange to stain cultured cells in a phenotypic screening of small-molecule compounds. This dye enabled clear and simultaneous distinction between the nucleus and cytoplasm with a single staining step, consistently delivering reliable results. In our imaging-based phenotypic analysis, CyTRAK Orange significantly simplified image processing compared to dyes that stain only the nucleus, as it provided precise spatial information on both the cytoplasm and nucleus within fluorescent images. Overall, CyTRAK Orange proved to be a convenient and dependable staining solution, making it an invaluable tool in our research.

Name: Junichi Sawada

Organisation: University of Shizuoka, Japan

.........................................

Manufacturer's response: Dear Junichi -san, Thank you for this generous review of CyTRAK Orange.  You have cleverly maximised the information that can be extracted from your cell-based assay and its seems CyTRAK Orange played a useful part in that!

"good lipid stain"

PRODUCT:                 DRAQ9
Application Area:        Short-term membrane staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 DRAQ9 was a gift from BioStatus. We used it to determine if the vacuoles that we were observing in mammalian cells under hypotonic stress are surrounded by a lipid membrane. DiI that we tried before failed to stain the vacuoles, but with DRAQ9 the result was unequivocally positive: a bright staining was easily detectable under a confocal microscope. The presence of lipid was confirmed with Nile Red. We published the image in Fig. 5 of https://www.nature.com/articles/s41598-024-79815-z.pdf

Name: Michael Model

Organisation: Kent State University

"very good and easy to work with"

PRODUCT:  DRAQ7
Application Area:  Biochemistry

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.7

Comments:  Very good and easy to work with. We use it in almost all projects in our lab.

Name: Rodrigo Oliveira
Organisation: Universidade de São Paulo, Faculdade de Odontologia de Bauru
Review Date: 28 Nov 2024

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"easy and useful"

PRODUCT:              DRAQ5

Application Area:     Neuroscience, confocal microscopy

Ease of Use:             ⭐⭐⭐⭐⭐
After Sales Service:  ⭐⭐⭐⭐⭐
Value for Money:       ⭐⭐⭐⭐
Average rating:           4.7

Comments:               It's easy to identify the target, and the signal is strong enough to capture the image.

Name: Hao-Jung Cheng

Organisation: National Sun Yat-Sen University

"molecular biology"

PRODUCT:               DRAQ5
Application Area:      Confocal Microscopy

After Sales Service:  ⭐⭐⭐⭐⭐

Value for Money:      ⭐⭐⭐⭐⭐

Ease of Use:              ⭐⭐⭐⭐⭐

Average rating:          5.0

Comments:                Easy to use and highly productive 

Name: Sanaa Bendahmane

• Organisation: Private University of Marrakech

"Works as expected"

PRODUCT:                 DRAQ5

Application Area:        DNA labeling for nano-imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It is easy to use. The photo-stability and brightness are great. The far-red spectrum also provides less potential damages to DNA structures.

Name: Wing Shun Li

Organisation: Applied Physics Program, Northwestern University

"Good"

PRODUCT:                  DRAQ5

Application Area:        Immunofluorescence

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Works as expected

Name: Nahla Ibrahim

Organisation: Medizinischen Universität Wien

"very good"

PRODUCT:  DRAQ7
Application Area:  Cell death

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐⭐ (5.0)
Average Rating:	5.0

Comments:  A good far-red dye for cell death.

Name: Shashank Dadsena
Organisation: University of Cologne
Review Date: 12 Jun 2024

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"a very easy to prepare and use product that delivers accurate, reliable results"

PRODUCT:  DRAQ7
Application Area:  Analyze cell viability in compound screenings

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐ (4.0)
Value for Money:	⭐⭐⭐⭐⭐ (5.0)
Average Rating:	4.7

Comments:  I use DRAQ7 for cell staining to enable a precise and reliable quantification of the viability of my cells before and after the application of compounds at varying concentrations. It's very easy to use with no need to suspend the stock solution, and readouts of the nuclear stain are always crisp! I have never had any problems with the reproducibility of results, with clear and precise control values every single time. There is honestly nothing bad I can write about this product as I have never had any problems, and it's so simple to use. I do not personally purchase this product, but my team has never had any problems with ordering and delivery, with fast turnaround times.

Name: Kieran Feltham
Organisation: Anglia Ruskin University
Review Date: 22 May 2024

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"Very simple use to detect the nuclei and the signal is generally bright"

PRODUCT:                 DRAQ5

Application Area:        To visualize nuclei for basic research

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: It is easy to use and the staining is generally bright. In my case it was helpful since it was not possible to detect the fluorescence released by other compounds used to visualize nuclei such as dapi or hoechst 33342.

Name: Raffaele Strippoli

Organisation: Sapienza University (Rome, Italy)

"Great results!"

PRODUCT:                 DRAQ5

Application Area:        Confocal microsopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: The product is highly effective for confocal microscopy use and easy to use. We could achieve high-quality and reproducible results. Quality meets the value of money, also considering the after-sales care.

Name: Flavia Trionfetti

Organisation: IRCCS INMI Spallanzani

"Great Result!"

PRODUCT:                 DRAQ5

Application Area:        Staining Nuclei

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The staining result was of high quality.

Name: Julie Bejoy

Organisation: Vanderbilt University Medical Center

"Great nuclear counterstain"

PRODUCT:                 DRAQ5

Application Area:        Nuclear counterstaining in fluorescence microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: DRAQ5 is a bright and easy-to-use nuclear counterstain in the red part of the spectrum. It doesn't require washing after incubation and hence can save you a couple of minutes in the lab.

Name: Kseniia Sarieva

Organisation: Hertie Institute for Clinical Brain Research

"A bacterial DNA DRAQ5 was added to differentiate them from the background."

PRODUCT:                  DRAQ5

Application Area:        labeling bacterial viability for flow cytometry and confocal microcopy analysis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We ordered the product from Thermo Fisher. It is very easy to use in an effective and economical concentration range (after titration). Its use on fixed bacteria is a success. 

The protocol we have developed is the subject of a technical note: A flow cytometry method for safe detection of bacterial viability (DOI: 10.1002/cyto.a.24794)

Name: Sophie Servain Viel

Organisation: plateforme CYM - UMS IPSIT (Paris)

"good for necrotic nuclei, pity that Draq5 and Draq9 are at the same wavelengths"

PRODUCT:  DRAQ7
Application Area:  Visualize necrosis on glioblastoma primary spheroids with LSFM imaging

Ease of Use:	⭐⭐⭐⭐ (4.0)
After Sales Service:	⭐⭐⭐⭐ (4.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.0

Comments:  Glioblastoma spheroids of ~1mm in diameter are incubated with DRAQ7 for 16-20hrs before imaging. DRAQ7 is visualized in far red and stains dead nuclei, without discriminating cell death patterns. Easily combined with blue and green probes. **Photobleaches after 6hrs**.

Name: Mariam-Eleni Oraiopoulou
Organisation: Foundation for Research and Technology - Hellas
Review Date: 14 Sept 2023

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"great results"

PRODUCT:  DRAQ7
Application Area:  Cell viability assessment

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.7

Comments:  The dye is perfect for detecting dead cells by flow cytometry. Stable, easy to use, quite sensitive.

Name: SHANSHAN DU
Organisation: University of Galway
Review Date: 13 Sept 2023

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"Great results"

PRODUCT:                 DRAQ5

Application Area:        Cell cycle analysis of leukemia cells

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: We have obtained excellent results staining acute myeloid leukemia cells for flow cytometric analysis of the cell cycle.

Name: Chiara Boggio

Organisation: Università del Piemonte Orientale

"Great and reliable results, easy to use, recommended in IF experiments"

PRODUCT:                  DRAQ5

Application Area:        analyze tissue samples and cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: DRAQ5 is easy to use and provides reliable and consistent  staining, when the datasheet information is followed. High-quality results are achieved in confocal microscope in immunofluorescence experiments using cells or tissues.

Name: Pinelopi Kastana

Organisation: University of Patras

"Great result"

PRODUCT:                 DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use. Just followed the manufacture's protocol and got great microscopy images.  To lower the autofluorescence which we observed with Hoechst33342 on certain materials, we chose DRAQ5 and could get clear nuclear staining images.

Name: Sachiko Imaichi

Organisation: MGH

"great reproducible results"

PRODUCT:  DRAQ7
Application Area:  Assess nuclei in 2D and 3D immunofluorescence

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐⭐ (5.0)
Average Rating:	5.0

Comments:  We use this product to look at nuclei in both 2D and 3D fixed immunofluorescence imaging. Very useful if you do not have access to a 405 laser and cannot use DAPI, as this uses the 647 channel.

Name: Danielle Buglak
Organisation: UNC Chapel Hill
Review Date: 4 May 2023

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"It is easy to use and reliable as an internal control"

PRODUCT:                 DRAQ5

Application Area:        In cell western blot (in neural tube coronal slices)

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.3

Comments: The product is ideal as an internal control for immunofluorometric quantification using immunofluorescence (infrared range). Results are reproduced consistently.

Name: Anayansi Molina-Hernández

Organisation: INPer, Mexico  

"Very easy to use. High-quality results"

PRODUCT:                  DRAQ5

Application Area:        Cell staining for flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: Very easy to use. High-quality results

Name: Francesco Bellanti

Organisation: University of Foggia

"Great results and publication!"

PRODUCT:                  DRAQ5

Application Area:        Proliferation of cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use, nice results!

Name: Stéphane Schurmans

Organisation: University of Liège

"The results were good, but DraQ5 was too expensive, its utilization was limited."

PRODUCT:                  DRAQ5

Application Area:        detect the cell nuclei in confocal fluorescent microscopy

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: The product worked well. Our main problem with these stains is that they cannot be used in any microscope, e.g. Draq5 can be used in a confocal laser microscope, but cannot be used in another, simpler microscope, so we have to purchase different stains for different microscopes. In addition, DraQ5 is more expensive than other similar stains.

Name: Andras Hrabak

Organisation: Semmelweis University

.........................................

Manufacturer's response: Thank you for your review of DRAQ5. You are right that few epifluorescence microscopes have far-red detection capabilities. You may wish to explore the use of CyTRAK Orange which is excited optimally at 510 nm (488 ex. compatible) and with peak emission at 615 nm (“rhodamine" channel). We do appreciate your concern about pricing.. sadly we have no legal control over end-user pricing by our channel partners. You might, however, consider purchasing direct from BioStatus for the keenest price, fastest service and our expert technical support.

"Great results!"

PRODUCT:                  DRAQ5

Application Area:        Analyze nucleus in confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: This product is very simple to use and gives high quality result. It also guarantees reproducibility of experiments. I used it for nucleus staining in confocal microscopy at 1:1000 concentration.

Name: Giulio Bontempi

Organisation: University La Sapienza of Roma

"An easy-to-use DNA dye suitable for tissue clearing of mouse brain samples"

PRODUCT:                  DRAQ5

Application Area:        Fluorescence microscopy counterstain for tissue clearing samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: The DraQ5 fluorescent probe is supplied as a water-soluble concentrated solution (0.5mM or 5mM) that can be diluted depending on the desired application. Given its high affinity for double-stranded DNA, DraQ5 can be used as a nuclear counterstain in immunohistochemistry studies upon short incubation periods of time, as an alternative to blue-fluorescent DNA-binding probes. However, the broad excitation and emission peaks must be taken into account if a combination with other fluorophores is desired. In our experience, it is also compatible with aqueous-based tissue clearing techniques, rendering a uniform labelling across entire mouse brain hemispheres (55-days-old) that could be imaged by light-sheet fluorescence microscopy. To achieve this, a simple incubation at 37⁰C/60rpm for 12h with a DraQ5 diluted solution (2.5uM) and a 12-hour washing step at 4⁰C to remove excess were incorporated in the tissue clearing procedure.

Name: Miguel Lopes

Organisation: Center for Neuroscience and Cell Biology (CNC)

"excellent product to safely immobilise motile cells"

PRODUCT:                 CyGEL
Application Area:        Live cell fluorescent imaging of Leishmania promastigotes

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments:                 Without Cygel it would be almost impossible to obtain high-quality microscopy of motile forms of Leishmania parasites. The product is easy to use and gives a reproducible performance. I've used it to image Leishmania promastigotes that were modified to contain fluorescent proteins, in order to gain information on protein function. Cellular morphology remained unchanged by the embedding process and protein localization was not affected. Can highly recommend Cygel.

Name: Nathaniel Jones

Organisation: University of York

"Great result"

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.3

Comments: The product was easy to use. We achieved high-quality results. We took excellent photos with the confocal microscope. The results were reproducible

Name: Sara Shayan

Organisation: Pasteur Institute of Iran

"It is ok"

PRODUCT:                  DRAQ5

Application Area:        Cancer imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The effect is ok, but not very stable

Name: Zi Wang

Organisation: CAS

.........................................

Manufacturer's response: Dear Zi, It would be helpful to understand what the issue was in more detail. If staining is not stable over time that can be the case in live cells, where after several hours a re-distribution can occur in some cell types, mostly towards lipophilic structures in the cytoplasm (Martin, 2005). If the staining is not stable in fixed cells then this may be due to washing post-staining, too low concentration, or not removing surfactants used in prior cell permeabilisation procedures.

"great results"

PRODUCT:  DRAQ7
Application Area:  Analyze ROS on tumor cells

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.7

Comments:  Product is simple and effective. We intend using again.

Name: Rodrigo Oliveira
Organisation: Universidade de São Paulo
Review Date: 17 Jun 2022

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"good results"

PRODUCT:  DRAQ7
Application Area:  Flow cytometry

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.7

Comments:  Easy to use. Reliable results.

Name: Michal Simon
Organisation: Kaplan Medical Center
Review Date: 3 May 2022

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"it works well, minimal crosstalk with other fluorescent channels"

PRODUCT:  DRAQ7
Application Area:  Nuclear counter stain in deep red

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐ (4.0)
Average Rating:	4.7

Comments:  It works well, minimal crosstalk with other fluorescent channels. If you have weak signal, use a higher concentration than recommended.

Name: Joshua Morgan
Organisation: University of California, Riverside
Review Date: 21 March 2022

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"Easy to use. Nice results"

PRODUCT:                  DRAQ5

Application Area:        Detection of nuclei in CUBIC cleared samples for light sheet microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: Easy to use. Nice results

Name: Martin MAjer

Organisation: Charles University

.........................................

Manufacturer's response: Thank you so much for the product review, Martin. We value this feedback! We would like to understand how we can do better at after-sales service as we have no record of a direct enquiry, but understand that there maybe at-arms-length improvements to undertake.

"Excellent nuclear stain. Easy to use. Works well with other common stains"

PRODUCT:                  DRAQ5

Application Area:        Cell Staining (nuclei/DNA)

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is simple to use since a washing step is not required. It provides a strong signal and good visualization of cell nuclei when used in confocal laser scanning microscopy (CLSM). Its red fluorescent spectrum makes it a suitable stain to work with in conjunction with other common blue and green stains as signal overlap can be avoided.

Name: Joachim Kjesbu

Organisation: Norwegian University of Science and Technology

"Great product and highly reliable"

PRODUCT:                  DRAQ5

Application Area:        Intravital fluorescence microscopy in immune reaction observations

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is perfect to be used in in vivo applications. It allowed cell nuclei segmentation from intravital fluorescence images. OK both in mammalians and chicks.

Name: Claudio Conci

Organisation: Politecnico di Milano, Dip Chimica Mat e Ing. Chimica G.Natta

"Far red cell nucleus stain"

PRODUCT:                  DRAQ5

Application Area:        Cell colocalization analysis

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐
Average rating:          2.7

Comments: DRAQ5 is generally easy to use. The spectra are in long wavelength that can be paired with many other fluorophores and thus our choice of nucleus dye for confocal imaging. However, the performance is not very stable, sometime it can be very dim and unspecific staining outside the nucleus can be seen. The product (from Thermo Fisher) is very expensive. It will be good if the price can be more reasonable.

Name: Yuning Hong

Organisation: La Trobe University

.........................................

Manufacturer's response: Dear Yuning, thank you for detailed review of DRAQ5. Without seeing examples of the issues you raise it is difficult to give further technical advice. The occasional cytoplasmic staining you describe is often a symptom of understaining or washout due to residual surfactant left over from a prior washing step. Regarding cost, there are a number of other carefully selected suppliers you can choose from and we can advise on the most competitive of these or alternatively order direct from us, the source!

"great results"

PRODUCT:  DRAQ7
Application Area:  Flow cell sorting

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐⭐ (5.0)
Average Rating:	5.0

Comments:  This product works very well for flow cell sorting.

Name: Chaoqun Wang
Organisation: UCSF
Review Date: 4 January 2022

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"Compared with other nuclear dyes, this is one of the best, at least, in our hands"

PRODUCT:                  DRAQ5

Application Area:        Budding yeast nuclear staining of live cells observations

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: DRAQ5 is very easy to use. You resuspend cells in PBS, stain cells in dark for 1-3 minutes, then you can image cells directly (no rinsing is needed). The product is kind of expensive, but it is worth it. The dye can be excited by either 488 or 639 nm laser, but emits in far red, so it won't interfere with GFP.

Name: Zhaojie Zhang

Organisation: University of Wyoming

"Great results"

PRODUCT:                 DRAQ5

Application Area:        Cells and Tissue imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We achieved high quality and reproducible data.

Name: Cindrilla Chumduri

Organisation: University of Würzburg

"Excellent reproducible results."

 PRODUCT:                 DRAQ5

Application Area:        Fluorescently paint cell nucleus

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The DRAQ5 reagent is very easy to use. I have used it together with DAPI for counterstaining the cellular nuclei in various cell types like SHSY-5Y, SKN-SH, U2OS and HeLa. The reagent is robust and the staining is perfectly reproducible. I had used the probe in a variety of application like Fluorescent Microscopy, FISH and combined FISH with ICC. The probe lifetime is commendable even after multiple exposures. You can find images with application of DRAQ5 in my publication here - Chanda, K., Jana, N.R. & Mukhopadhyay, D. Receptor tyrosine kinase ROR1 ameliorates Aβ1–42 induced cytoskeletal instability and is regulated by the miR146a-NEAT1 nexus in Alzheimer’s disease. Sci Rep 11, 19254 (2021). https://doi.org/10.1038/s41598-021-98882-0

Name: Kaushik Chanda

Organisation: The Scripps Research Institute, Florida