Nuclei of mouse spleen cells were stained with Draq5 dye (1:500) (Abcam, Cambridge, UK) and slides were mounted into ProLong ®Gold Antifade Reagent (Cell Signaling Technology, MA, USA).

PRODUCT:                  DRAQ5

Application Area:        Immunofluorescence microscopy

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Comments: To our experience, the best staining was achieved when dye Draq5 was added to cells together with the fluorescent secondary antibody  and incubated at least 1 hr at room temperature.

Name: Gabriela Hrčková

Organisation: Anon., Slovakia

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Manufacturer's response: Dear Gabriela, thank you for your technical feedback on the use of DRAQ5 in immunofluorescence methods - combining antibodies and DRAQ5 is a nice way to streamline a procedure.