"Good results, bio-safe, easy to use, reliable, helped in visualization of nuclei in live HeLa cells and calculation of Nuclear vs Cytoplasmic ratio of LC3 using MATLAB."

PRODUCT:                 DRAQ5

Application Area:       Nuclei visualization in live HeLa cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 was used to visualize nuclei in live HeLa cells using confocal fluorescence microscope LSM 510. This product is very stable and convenient to use. The protocol involving dilution and staining is very simple and easy to follow. The dye is not toxic to HeLa cells and bio-safe. The dye did not interfered with subsequent downstream biochemical analysis e.g. western blot and protein band detection by LI-COR.  DRAQ5 uses far red wavelength, it does not involve the risks associated with UV light as associated with some other nuclear staining dyes in market. Also it was advantageous to us since nuclei visualization was the most essential part of our experiments and DRAQ5 imaging was compatible with our LSM 510.

Name: pallavi manral

Organisation: Vanderbilt University