Monday, 19 September 2016

"DRAQ7 is an easy to use viability dye that allows monitoring of cell death over time."

PRODUCT:                 DRAQ7

Application Area:       Cell viability

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3


Comments: DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment.  
Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes.  We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible.

Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation.

Name: 
Daphné Laubreton

Organisation: INRA

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