PRODUCT: DRAQ7
Application Area: Cell viability
Ease of Use: ⭐⭐⭐⭐⭐
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Average rating: 4.3
Name: Daphné Laubreton
Average rating: 4.3
Comments: DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment.
Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes. We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible.
Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation.
Organisation: INRA
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