"Great results"

PRODUCT:                  DRAQ5

Application Area:        To analyze nuclei in the cultured isolated bovine chondrocytes

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.0

Comments: The dye was highly effective as even at very diluted concentrations produced excellent results. The confocal images obtained from these chondrocytes whose nuclei were labelled with DRAQ5 produced high quality and reproducible results. I had used this dye to count the number of cells in clusters because in my culture experiments the cell membranes were frequently touching and therefore thresholding of individual cells was impossible. In order to count number of cells in clusters I counted number of nuclei and DRAQ5 gave me highly reliable results as I could count the number of nuclei in 3D by using Volocity (a 3D image analysis software). It is quite an expensive dye but as the very diluted concentrations are very effective therefore, justifies the cost. Moreover, it was easy to use and highly penetrable and required very little time to label nuclei efficiently.

Name: Asima Karim

Organisation: The University of Edinburgh

"Nice alternative to DAPI."

PRODUCT:                  DRAQ5

Application Area:        analyse transplanted tissue

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.7

Comments: Fairly easy to use. Need to be made clear not to wash slides after DRAQ5 staining or colouring will be lost.

Name: Sarah Pringle

Organisation: UMCG 

.........................................

Manufacturer's response: Dear Sarah, Thank you for your helpful reminder. We do indeed emphasise on our official technical datasheet that one should use DRAQ5 last and refrain from washing slides thereafter; if necessary any excess liquid can be tamped away. Kind regards, Roy Edward Sales & Marketing Director

"Great and reliable results"

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining in fixed cell

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is very easy to use. The results are very good and reliable in PFA and methanol fixed cell,. The fluorescence signal is of high qualitiy.

Name: Sabrina Ernst

Organisation: Institute of Biochemistry and Molecularbiology

"It gives us great results, thanks."

PRODUCT:                  DRAQ5

Application Area:        Analyze reactive oxygen species in cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and well-fit for your purpose.

Name: Seung Hyo Lee

Organisation: KAIST 

"Great and dependable nuclear dye"

PRODUCT:                  DRAQ5

Application Area:        Immunofluoresence

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product is very easy to use. Gives great reproducible nuclear staining. We have used this product both in immunofluorescence as a subcellular localisation marker and as a reference dye for plate based assays using a fluorescent reader (In-cell ELISA, ...).

Name: Luc Bertrand

Organisation: University of Miami School of Medicine

"Reliable and sensitive DNA stain"

PRODUCT:                  DRAQ5

Application Area:        immunofluoresence high throughput image analysis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: We found this product reliable and very easy to use. It worked consistently and we were very happy with its application. The product is quite expensive but we found we were able to use it at a lower concentration than recommended with just as great results.

Name: Hayley Newton

Organisation: University of Melbourne

"A very useful product for live cell imaging."

PRODUCT:                  DRAQ5

Application Area:        Microscopy

Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐

Value for Money:        ⭐⭐⭐
Average rating:           2.3

Comments: The dye gives strong staining and could we washed away even from live cells. Nuclei of some cell lines e.g. PC3 could not be stained (the dye does not enter the cells) others e.g H1299 contain strange dotty staining in the cytosol in addition to the nuclear staining. Too high concentration causes artificial condensation of chromatin.

Name: Rastislav Dzijak

Organisation: Institute of organic chemistry and biochemistry

"Works well for cell proliferation assays"

PRODUCT:                  DRAQ5

Application Area:        Quantify cell numbers in response to small molecules in a high-throughput format

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: This product allowed us to perform screens of compounds on cells that could not be assayed using traditional cell proliferation assays like Alamar Blue or MTT. The dye allows rapid quantification of cell numbers in a 96-well plate format. Assay of wells with multiple cell doses generated nice curves that gave us confidence in the quantitative ability of the assay over a wide dynamic range of cell numbers.

Name: Laurie Ailles

Organisation: University Health Network

"Very useful! Using it a lot."

PRODUCT:                  DRAQ5

Application Area:        DNA staining for high-throughput imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Great staining and consistent results. Have used DRAQ5 for the past 5 years.

Name: Diana Stavreva

Organisation: NCI

"Easy to use visualization of DNA"

PRODUCT:                  DRAQ5

Application Area:        stain DNA in living cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Easy to use, good brightness with limited photobleaching and phototoxicity

Name: Claire Walczak

Organisation: Indiana University

"Great and reliable results. Highly recommended."

PRODUCT:                  DRAQ5

Application Area:        To demonstrate binding of extracellular vesicles to leukocytes

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ-5 is easy to use and highly reproducible. it is a go-to reagent for nuclear identification.

Name: Joel Pachter

Organisation: UConn Health

"very useful chemical"

PRODUCT:                  DRAQ5

Application Area:        Analyze cell cycle and otehr parameters in cultured cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Excellent results with this consumable, we use it routinely

Name: Jyotsna Dhawan

Organisation: Institute for Stem Cell Biology and Regenerative Medicine 

"Great reagent, definitely worthy to be purchased and used!"

PRODUCT:                  DRAQ5

Application Area:        Confocal microscopy on Breast Tumor and Lung metastases sections

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a valuable alternative to DAPI if you want to use a red fluorescent dye for staining your nuclei. Its  low-photobleaching, easy to use and yields a low background noise.

Name: Cristina Andreani

Organisation: University of Camerino, MC, Italy (Now @ UTSouthwestern Medical Center, Dallas, Texas) 

"Good staining and good tools for IHF/IF"

PRODUCT:                  DRAQ5

Application Area:        Visualize nuclei in cells and tissus samples

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.7

Comments: DRAQ5 is easy to use and fast (30min of incubation) and it stains well.

Name: chloé terciolo

Organisation: INSERM