"Great results"

PRODUCT:                  DRAQ5

Application Area:        To analyze nuclei in the cultured isolated bovine chondrocytes

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.0

Comments: The dye was highly effective as even at very diluted concentrations produced excellent results. The confocal images obtained from these chondrocytes whose nuclei were labelled with DRAQ5 produced high quality and reproducible results. I had used this dye to count the number of cells in clusters because in my culture experiments the cell membranes were frequently touching and therefore thresholding of individual cells was impossible. In order to count number of cells in clusters I counted number of nuclei and DRAQ5 gave me highly reliable results as I could count the number of nuclei in 3D by using Volocity (a 3D image analysis software). It is quite an expensive dye but as the very diluted concentrations are very effective therefore, justifies the cost. Moreover, it was easy to use and highly penetrable and required very little time to label nuclei efficiently.

Name: Asima Karim

Organisation: The University of Edinburgh