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PRODUCT:                  DRAQ5

Application Area:        Visualize nuclei using confocal microscopy

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Comments: We used DRAQ5 to label the nuclei of live adult rat ventricular myocytes (see Weeks et. al. β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. Journal of the American Heart Association. 2017 6(4)). We labelled the cells with 1 uM DRAQ5 for 10 minutes prior to imaging, which was lower than the recommended concentration, but it fluoresced nicely.

Name: Kate Weeks

Organisation: Baker Heart and Diabetes Institute