Wednesday, 28 June 2017

"Good nuclear stain that fluoresces in the far red."

PRODUCT:                  DRAQ5

Application Area:        High content imaging.


Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐
Average rating:           3.3


Comments: DRAQ5 is great as it can be used to stain living cells as well as fixed ones.  Of course, the cells won't  divide in it, so you can't use it for long-term time-lapse imaging, but it's still very useful for shorter time lapses.

Name: 
Bilal Zulfiqar

Organisation: Griffith University

.........................................

Manufacturer's response: Dear Bilal, thanks for your helpful feedback on DRAQ5. This is important information – we do not recommend DRAQ5 for long-term exposure to live cells as it, like ALL high affinity DNA binders and intercalators, will have some kind of impact on cell health. There are exceptions especially with terminally-differentiated cells as observed with HUVEC, macrophages and BaF3. However, short-term exposure and use in live-endpoint assays has been a hallmark of the product’s success in drug discovery and flow cytometry applications, in particular the loss of cytoplasmic GFP signals when cells are partially permeabilized by fixation with formaldehyde and the unspecific loss of cells due to RBC lysis and associated washes, respectively.


Monday, 19 June 2017

"Perfect for our needs, and gave consistent results."

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining of primary fibroblasts


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7


Comments: Product was straight forward to use, fast and consistent. Perfect for the application we required as a counter-stain for immunofluorescence using green and red fluorophores.  Product lasted a long time (if stored correctly) and gave robust results until the last drop. The instructions which came with the product were straight forward and easy to follow for consistent results.

Name: 
Keith Wheaton

Organisation: University of Ottawa

"Great results"

PRODUCT:                  DRAQ5

Application Area:        Implant fabrication


Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐
Average rating:           3.7


Comments: Good product/ fast delivery/ in general i do recommend it to people in the field

Name: 
A Kiani

Organisation: UNB

Wednesday, 7 June 2017

''I loved the images obtained from the DRAQ5"

PRODUCT:                  DRAQ5

Application Area:        in vitro aplications


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0


Comments: We had very nice results using DRAQ 5 and we have published recently a paper. The price was a bit high.

Name: 
Diaconeasa Maria

Organisation: Universitatea de Ştiinţe Agricole şi Medicină Vete

Monday, 5 June 2017

"Easy to use dye for analysis of DNA content without compensation issues in flow."

PRODUCT:                  DRAQ5

Application Area:        immunology, B-cell development and leukemia


Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0


Comments: Easy to use, good staining, no compensation issues in flow cytometry.

Name: 
Vincent van der Velden

Organisation: Erasmus MC, Immunology