"Good nuclear stain that fluoresces in the far red."

PRODUCT:                  DRAQ5

Application Area:        High content imaging.

Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.3

Comments: DRAQ5 is great as it can be used to stain living cells as well as fixed ones.  Of course, the cells won't  divide in it, so you can't use it for long-term time-lapse imaging, but it's still very useful for shorter time lapses.

Name: Bilal Zulfiqar

Organisation: Griffith University

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Manufacturer's response: Dear Bilal, thanks for your helpful feedback on DRAQ5. This is important information – we do not recommend DRAQ5 for long-term exposure to live cells as it, like ALL high affinity DNA binders and intercalators, will have some kind of impact on cell health. There are exceptions especially with terminally-differentiated cells as observed with HUVEC, macrophages and BaF3. However, short-term exposure and use in live-endpoint assays has been a hallmark of the product’s success in drug discovery and flow cytometry applications, in particular the loss of cytoplasmic GFP signals when cells are partially permeabilized by fixation with formaldehyde and the unspecific loss of cells due to RBC lysis and associated washes, respectively.