"Nice reagent for cell cycle analysis"

PRODUCT:                  DRAQ5

Application Area:        Analysis of cell cycle status in colorectal cancer cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a useful reagent for analysing the cell cycle status of live/dead cells by flow cytometry. For our purposes, cells were first killed by fixation in 70% Ethanol  (because we were co-staining for other antigens) and eventually resuspended in buffer containing 2ul/ml DRAQ5 allowing 10 mins prior to flow cytometric analysis. Lovely DNA profiles were obtained with no compensation issues with the other fluorochrome (PE) used in the study. Never attempted on live cells but we understand from other colleagues in the department that this is possible. Care must be taken when handling as this is a toxic substance.

Name: Rhys Morgan

Organisation: University of Bristol