"Great results"

PRODUCT:                 DRAQ5

Application Area:       Protein translocation using image stream

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: The nucleus staining is very easy to use. It was stained 1:1000 in PBS Buffer and analysed by Amnis image stream

Name: Juliane Klein

Organisation: Institut für Immunolgie

"Absolutely necessary to document movement of iron through intestinal cells"

PRODUCT:                 DRAQ5

Application Area:       Analysis of metabolites within cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Extremely easy to use.  Highly reproducible results.  Easy to quantify.

Name: Jonathan Glass

Organisation: Feist-Weiller Cancer Center, LSU Health Sciences Center

"Excellent DNA dye - we use for single staining in antiparasitic high content assays"

PRODUCT:                 DRAQ5

Application Area:       Fluorescence microscopy in high content screening

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: Ideal for single-staining high content assays, DRAQ5 enables clear visualization of cell nucleus and gives a  smoother background signal at cell cytoplasm - which is enough for image processing softwares to delimitate and segment cellular structures properly. Moreover, the stain stability enables material storage for longer period than other conventional dyes (we have imaged 384-well plates after 4 weeks post-staining and the fluorescence signal was sufficiently high); also there is no need for dye washout after staining the microplates (less pipetting stesp). We recommend DRAQ5 in single-staning high content screening for assessing compound activity against intracellular trypanosomatids (Trypanosoma cruzi and Leishmania spp.) - the dye marks both DNA material from intracellular parasites and host cells - and providing a faint signal in the cytoplasm - turning it easy to segment and quantify the intracellular parasites.

Name: Caio Haddad Franco

Organisation: Instituto Butantan

"Excellent cell imaging pictures"

PRODUCT:                 DRAQ5

Application Area:       Checking interactions between cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This dye helps us to check synapsis between mast cells and pancreatic cancer cells. These findings provide evidence for a novel mode of unidirectional crosstalk between MCs and cancer cells implicating direct activation by cancer cells in MC reprogramming into a pro tumorigenic profile

Name: Gorzalczany Yaara

Organisation: Tel Aviv University 

"This is a product I intend to use, for as long as they produce it."

PRODUCT:                 DRAQ5

Application Area:       HIV infection and signaling cascades in cell samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a simple, easy-to-use product. Either used on live-primary or fixed cells a simple 5-10 minute incubation followed by washing steps produced consistent, repetitive results. Its fluorescent excitation/emission spectra are very well suited to combine with other fluorophores without channel bleeding or crosstalk. The particular spectral characteristics of DRAQ5 make it also easy to work with in fluorospectrometry-based experiments. We have  also used DRAQ5 combined with metabolic biomarkers in solution without observing interference with cellular metabolic uptake in short-time uptake experiments, this allowed us not only to reduce experimental time, but also helped us reduce cell manipulation.

Name: VICTOR CASTRO VILLELA

Organisation: UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE

"Easy way to add valuable data to all our experiments"

PRODUCT:                 DRAQ7

Application Area:       Monitor cell viability

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Great value, straightforward way to assess cell viability through flow cytometry in all our experimental settings. Easily incorporated into the work flow to provide robust and easy to interpret data.

Name: Cathy Thornton

Organisation: Swansea University