"Excellent DNA dye - we use for single staining in antiparasitic high content assays"

PRODUCT:                 DRAQ5

Application Area:       Fluorescence microscopy in high content screening

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: Ideal for single-staining high content assays, DRAQ5 enables clear visualization of cell nucleus and gives a  smoother background signal at cell cytoplasm - which is enough for image processing softwares to delimitate and segment cellular structures properly. Moreover, the stain stability enables material storage for longer period than other conventional dyes (we have imaged 384-well plates after 4 weeks post-staining and the fluorescence signal was sufficiently high); also there is no need for dye washout after staining the microplates (less pipetting stesp). We recommend DRAQ5 in single-staning high content screening for assessing compound activity against intracellular trypanosomatids (Trypanosoma cruzi and Leishmania spp.) - the dye marks both DNA material from intracellular parasites and host cells - and providing a faint signal in the cytoplasm - turning it easy to segment and quantify the intracellular parasites.

Name: Caio Haddad Franco

Organisation: Instituto Butantan