Thursday, 14 June 2018

"Draq5 is a great live cell nuclear stain"

PRODUCT:                 DRAQ5

Application Area:       Airway cell nuclei stain


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0


Comments: Draq5 is an easy to use and affordable live cell stain.

Name: 
Derek McMahon

Organisation: Univ of Pennsylvania

Tuesday, 12 June 2018

"Reproducible result, basic component for quantitative measurement of dead cells."

PRODUCT:                 DRAQ7

Application Area:       Quantitative measurement of dead cells in live cell imaging


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7


Comments: DRAQ7 works perfectly and always stain the nucleus of the dead cells. We use it each time to count the number of dead cells in live cell imaging. It works better than PI.

Name:
 Pankaj Deo

Organisation: Monash University

"Good product"

PRODUCT:                  DRAQ7

Application Area:        Analyze number of dead cells at each timepoint


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐
⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3


Comments: My colleagues and I could see a clear and bright signal even with low laser power. There was never an issue of poor dye penetration into 3D cellular aggregates, as would otherwise be observed with more common nuclear dyes, such as Hoechst . The quality of staining is usually consistent across experiments  and among different colleagues. This follows that our results were also consistent across experiments. The dye was stable after repeated uses, and the signal intensity of DRAQ7 did not decrease with repeated use. Moreover, as it is simply added to cell culture media, it fits easily into any experimental design.  I would recommend DRAQ7 to other researchers for its stability, fluorescence quality and its convenience of use. One suggestion for improvement would be to create versions in other fluorescence colors instead of just one which is presently APC.

Name: Sharon Lee

Organisation: Singapore-MIT Alliance for Research and Technology

"I am really happy with this product."

PRODUCT:                  DRAQ5

Application Area:        Flow cytometry immunology

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐
⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0


Comments: I am really happy with the use of this product allowing the elimination of dead cells for flow cytometry analysis.

Name:
 
Marion Humbert

Organisation: Université de Genève

Monday, 11 June 2018

"A useful far-red fluorescent dye for visualizing nuclei."

PRODUCT:                 DRAQ7

Application Area:       Cell counting in cell culture


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0


Comments: DRAQ7 is a useful product for those wishing to visualize nuclei in the far-red.  It was as simple to use as DAPI, but may have advantages over DAPI, as you don't need to use U.V. light to excite it.

Name: 
Paul Turko

Organisation: Charite University Medicine - Berlin

Saturday, 9 June 2018

"Provided exactly the requested staining. Great results!"

 PRODUCT:                 DRAQ7

Application Area:       Visualizing cell death in culture.


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0


Comments: We used DRAQ7 to visualize the formation of a necrotic core in fresh isolated Islets of Langerhans, which are prone to hypoxia.  DRAQ7 was simply added to the medium to a final conc. of 1 microM. This compound appeared specific for staining the nuclei of necrotic cells only. It is easy in use, provides a fairly low background and stable regarding bleaching.

Name: 
Kristmundur Sigmundsson

Organisation: Duke-NUS Medical School, Singapore

Friday, 8 June 2018

"Quick and easy to use stain."

PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.7


Comments: The product is very easy to use and gives accurate values for cell normalisation when compared to other nuclei stains. The use of draq5 in a plate reader really speeds up data acquisition.

Name: 
Oliver Russell

Organisation: Newcastle University

"Excellent product for use in live-cell applications."

PRODUCT:                 DRAQ5

Application Area:       Analyze protein subcellular compartmentalization in live cells


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0


Comments: We used this product to define nuclear localization of different proteins in cultured adult mouse cardiac myocytes. Draq5 was easy to use and provided a good fluorescent signal in the far-red spectrum that didn't interfere with our other fluorophores.

Name: 
Tim O'Connell

Organisation: University of Minnesota

"Reliable and robust product."

 PRODUCT:                 DRAQ5

Application Area:       Host-pathogen interactions - immunofluorescence


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐⭐
 Average rating:          4.0


Comments: Great results enabling us to reproducibly track Chlamydia trachomatis during its interaction with the host cell. Reliable and robust product.

Name: 
Richard Hayward

Organisation: University of Cambridge

"A must if using two or more reporters"

PRODUCT:                 DRAQ5

Application Area:       Cell cycle analysis


Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐
Value for Money:        ⭐⭐⭐
 Average rating:          3.3


Comments: It's very easy to use, with different fluorophores.

Name: 
Hardik Gala

Organisation: CCMB

"Good results and works fine for live-cells. For fixed cells I rather use DRAQ7."

PRODUCT:                 DRAQ5

Application Area:       Confocal microscopy


Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐
⭐⭐
Value for Money:        ⭐⭐⭐⭐
 Average rating:          4.3


Comments: DRAQ5 stain nuclei in an easy way, I have no doubt that is one of the best, mainly in live cells, though for the specific case of my lab we rather used cell-fixed preparations, though for this case DRAQ5 works fine as well. I'd recommend DRAQ7 which is a bit better. Cons, both of them are expensive in caparison with other nuclei staining dyes.

Name: 
Bruno Rivas-santiago

Organisation: Unidad de Investigacion Medica - Zactecas IMSS