PRODUCT: DRAQ5
Application Area: Nuclei staining
After Sales Service: ⭐⭐⭐⭐⭐
Average rating: 5.0
Organisation: Research Center Juelich GmbH
.........................................
Manufacturer's response: Thank you for your review of DRAQ5. DRAQ5 does have a useful weak secondary staining of the cytoplasm which HCS labs use to segment the cell envelope, by increasing the gain. An unwanted inversion staining (“hollow” nuclei) is a consequence of too low a concentration of DRAQ5. We strongly recommend 5 uM for fixed cells and 5-10 uM for live cells. We would be very happy to discuss this and troubleshoot the issue to try to resolve it.