Tuesday, 23 July 2019

"Great results! "

PRODUCT:                  DRAQ5

Application Area:        Nuclei staining


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐
⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0


Comments: Excellent and reliable staining of the nuclei! The only drawback is that sometimes it stains more than just the nuclei. It helped with the cell counting program.

Name:
 Rejhana Kolasinac

Organisation: Research Center Juelich GmbH

.........................................

Manufacturer's response: Thank you for your review of DRAQ5. DRAQ5 does have a useful weak secondary staining of the cytoplasm which HCS labs use to segment the cell envelope, by increasing the gain. An unwanted inversion staining (“hollow” nuclei) is a consequence of too low a concentration of DRAQ5. We strongly recommend 5 uM for fixed cells and 5-10 uM for live cells. We would be very happy to discuss this and troubleshoot the issue to try to resolve it.

Tuesday, 16 July 2019

"Standard nuclei/DNA counterstain in our lab for more than 15 years!"

PRODUCT:                  DRAQ5

Application Area:        DNA counterstain for immunofluorescence studies


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7


Comments: Super easy to use, just add it to your secondary antibody solution in immunofluorescence studies (cells and tissues), and you get reproducible stainings of your nuclei with excitation/emission wavelenghts that do not interfere with other common flurophores used in multiple immunofluorescence stainings.

Name:
 
Maren Rehders

Organisation: Jacobs University Bremen

Monday, 15 July 2019

"Very good."

PRODUCT:                  DRAQ5

Application Area:        HIV infection


Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐
Value for Money:        ⭐⭐⭐
Average rating:           4.3


Comments: The product is reliable, efficient and easy to use. It's results were very good.

Name:
 
Ran Taube

Organisation: BGU 

Sunday, 14 July 2019

"DRAQ7 is now implemented in our standard SVF cells staining protocol. "

 PRODUCT:                  DRAQ7

Application Area:        Detection of stromal vascular (SVF) cells isolated from lipoaspirate and microfragmented lipoaspirat  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐
⭐⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0


Comments: DRAQ7 is a very stable and easy-to-use dye. We carefully titrated dye using fix/perm approach after the staining for 7 surface markers. After optimization of the staining protocol we achieved strong signal with an easy discrimination of nucleated cell from the cellular debris and fat particles. Samples were analysed on Navios flow cytometer.

Name:
 
Denis Polancec

Organisation: Srebrnjak Children's Hospital