Monday, 6 July 2020

"Decent nuclear staining in far red channel."

 PRODUCT:                 DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐
⭐⭐⭐
Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0


Comments: If there is the need to visualize fixed nuclei by confocal microscopy in the far red, then Draq5 is the stain of choice. Of course it is not as bright as DAPI and probably the "gain" and "laser power" should be considerably increased. Also some leakage of staining in cytoplasm could be observed, but still it is very reliable for staining. For the best results, dilution 1:500 in 0.1M PBS (alternatively with 0.3% Triton X) should be used.

Name: 
Juraj Sevc

Organisation: Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Slovak Repub

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