"Assay for Measuring Viability in presence of AP and PA by DRAQ7TM staining"

PRODUCT:                 DRAQ7

Application Area:        Flow Cytometry

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    Not required

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: To test the effects APPA (10 µg/ml), AP (2.3 and 10 µg/ml) and PA (7.7 and 10 µg/ml) on T/C28a2 chondrocyte viability, DRAQ7TM (Thermo Fisher Scientific, Waltham, MA, USA) was used. Following 24 hours (h) of different treatments, cells were trypsinized and centrifuged at 1,500 rpm for 10 minutes (min). The harvested cells were suspended in 3 µM of the fluorescent dye DRAQ7TM (Thermo Fisher) and incubated in the dark, at room temperature (rt) for 10 min. The samples were run on CytoFLeX flow cytometer (Beckman Coulter Inc. Pasadena, Ca, USA). At least 1x104 cells per assay were measured. Data were analyzed using CyExpert V 2.5 software (Beckman Coulter Inc). Positive control cells were treated with 5 µM Eto plus 10 ng/µl OSM while negative control (Basal) represented cells without treatment.

Name: Mercedes Fernández Moreno

Organisation: INIBIC-GIR, Spain

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Manufacturer's response: Dear Mercedes, thank you for your review of DRAQ7.  We very much enjoyed your article, and the promising results to support further research on a pair of plant-derived phenolics to mitigate the impact of osteoarthritis. Fernández-Moreno, et al. Pharmaceuticals 18.9 (2025): 1386.