"Robust Nuclear Counterstaining with DRAQ5 in Live-Cell Confocal Imaging"

PRODUCT:                 DRAQ5

Application Area:        Live-cell fluorescence microscopy

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: In our work using live-cell confocal fluorescence microscopy, DRAQ5 was used as a nuclear counterstain to provide a clear and reliable cellular landmark during imaging experiments. The study focused on the intracellular localization of photoluminescent Ru(II) supramolecular assemblies, where accurate discrimination between cytoplasmic and nuclear signal was essential. DRAQ5 produced a strong, well-defined nuclear fluorescence signal that was spectrally resolved from the emission of the Ru(II) complex, enabling confident assignment of subcellular localization in live cells.

DRAQ5 performed consistently across experiments, displaying excellent membrane permeability and rapid nuclear staining without the need for fixation or permeabilization. Its high photostability allowed extended imaging sessions with minimal photobleaching. The relatively low-energy excitation wavelength reduced phototoxicity and cellular stress, preserving cell morphology and viability during live-cell imaging.

Overall, DRAQ5 proved to be an effective and reliable nuclear counterstain for multichannel live-cell fluorescence microscopy. Its high signal-to-noise ratio, compatibility with other chromophores, and minimal interference with photoactive metal complexes made it an ideal choice for our application. We would readily recommend DRAQ5 for studies requiring precise nuclear identification in live-cell imaging contexts.

Citation

Laisney, J.; Kriger, S. M.; Havrylyuk, D.; Unrine, J. M.; Heidary, D. K.; Glazer, E. C. Supramolecular Self-Assemblies of Ru(II) Phototherapeutics: Biological Activity of Micro- and Nano-Particles Acting as Reservoirs. J. Mater. Chem. B 2026, 10.1039.D5TB01785H. https://doi.org/10.1039/D5TB01785H.

Name: Sarah M Kriger

Organisation: NC State University, USA

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Manufacturer's response: Dear Sarah, thank you for the detailed review of DRAQ5.  This is a very interesting application area and others in the field of Ru(II) complex chemistry field should benefit from your experience.