"DRAQ5 and Retina"

PRODUCT:                 DRAQ5

Application Area:       Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:           5.0

Comments: This is an excellent easy to use marker of cell bodies, especially in retina and brain

Name: donld fox

Organisation: university of houston

"Nice images with confocal laser scanning microscopy"

PRODUCT:                 DRAQ5

Application Area:       Tumor cell lines

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: Worked with diluted stock solution in PBS (better handling and accuracy). The applied incubation time, temperature and concentration were important for good reproducibility. On permeabilized cells the nucleus had better signal and stained clearly.  On the non-permeabilized cells, the cytosol was stained also.

Name: Jozsef Muranyi

Organisation: MTA-TKI 

"Great data achieved, images clear"

PRODUCT:                 DRAQ5

Application Area:       Trafficking in cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: Very effective , very ease of use and one bottle can provide thousands data...

Name: Che Azurahanim Che Abdullah

Organisation: Universiti Putra Malaysia

"Great results and easy use."

PRODUCT:                 DRAQ5

Application Area:       See different nuclei cells types

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.0

Comments: The react is easy to use and very effective.  We can use it for different cells types and in different conditions.

Name: Marimar Encabo

Organisation: University of Zaragoza 

"Recommended"

PRODUCT:                 DRAQ5

Application Area:       Staining Nucleic Acids

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.7

Comments: We used DRAQ5 for staining of nuclear DNA - and realized that it also nicely stains RNA in Dictyostelium cells, which, for our purpose, was beneficial.

Name: Christian Hammann

Organisation: Jacobs University 

"DRAQ5 is vital to analyze each sample I process"

PRODUCT:                 DRAQ5

Application Area:       Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: I use DRAQ5 in order to separate living cells of dead cells in a suspension of brain tumor cells and to calculate the cellular DNA content. I use 3 ul of DRAQ5 3 minutes before the acquisition, therefore I spend several vials of DRAQ5 in a short time and it is an expensive dye.

Name: María González-Tablas

Organisation: Center for Cancer Research 

"Great result, applicable in all of our research"

PRODUCT:                 DRAQ5

Application Area:       Analyse dystrophin in tissue sample

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: High quality, reproducible, easy to use, high intensity

Name: Laetitia GUEVEL

Organisation: UMR703 PAnTher 

"Good nuclear stain"

PRODUCT:                 DRAQ5

Application Area:       Separate two populations based on DRAQ5+/-

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.7

Comments: Good alternative to Hoechst, DAPI for multiplexing. Stained cells must be washed multiple times to avoid dye leakage into unstained cells.

Name: J Wu

Organisation: MGH

"Good results, bio-safe, easy to use, reliable, helped in visualization of nuclei in live HeLa cells and calculation of Nuclear vs Cytoplasmic ratio of LC3 using MATLAB."

PRODUCT:                 DRAQ5

Application Area:       Nuclei visualization in live HeLa cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 was used to visualize nuclei in live HeLa cells using confocal fluorescence microscope LSM 510. This product is very stable and convenient to use. The protocol involving dilution and staining is very simple and easy to follow. The dye is not toxic to HeLa cells and bio-safe. The dye did not interfered with subsequent downstream biochemical analysis e.g. western blot and protein band detection by LI-COR.  DRAQ5 uses far red wavelength, it does not involve the risks associated with UV light as associated with some other nuclear staining dyes in market. Also it was advantageous to us since nuclei visualization was the most essential part of our experiments and DRAQ5 imaging was compatible with our LSM 510.

Name: pallavi manral

Organisation: Vanderbilt University

PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence - nuclei staining

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 is easy to use, gives reproducible results. Excellent product - good replacement of DAPI.

Name: Przemyslaw Juszczyński

Organisation: IHiT

"Bright signal. More consistent and lower spillover than DAPI."

PRODUCT:                 DRAQ5

Application Area:       Nuclear marker in localization studies (ImageStream), Tissue ImmunoFluorescence

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: We used DRAQ5 as nuclear staining in Flow Cytometry and ImageStream analysis to track the subcellular localization of different proteins. It works well with different detectors and offers a nice alternative to DAPI, leaving UV fluorophores available for other markers. 

This product is also very convenient in tissue immunofluorescence as a cellular marker (nuclei). It allows easy characterization of immune infiltrates in diverse tissues with better and more consistent signal than DAPI.

Name: Gerard Hernandez Mir

Organisation: University of Pittsburgh

"Perfect for nuclear staining!"

PRODUCT:                 DRAQ5

Application Area:       Molecular mechanisms of cell signaling and nuclear/cytoplasmic localization

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: I have been using this product for past 6 years and it is reliable, perfect and very easy to use.

Name: Yuvaraj Sambandam

Organisation: Medical Universtiy of South Carolina

"Great and reproducible results, I recommend the use of DRAQ5."

PRODUCT:                 DRAQ5

Application Area:       Staining of DNA in rat leukocytes

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: We used DRAQ5 staining for the validation of FSC/SSC dot plot based identification of rat leukocytes from lysed whole blood sample. The use of DRAQ5 is easy, it stained the nuclei instantly. We used DRAQ5 several times and achieved always similar staining intensities, indicating good reproducibility.

Name: Kornelia Szebenyi

Organisation: MTA TTK, Hungarian Academy of Sciences

"Very happy with this product."

PRODUCT:                 DRAQ5

Application Area:       Microscopy.

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: Very easy to use. Consistent nuclear staining for fluorescent microscopic analysis of cells and FFPE tissue.

Name: Jen Whiting

Organisation: University of Washington

"Excellent product that gives highly reproducible results."

PRODUCT:                 DRAQ5

Application Area:       Confocal microscopy looking at viral replication in neuronal cells in culture.

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: We used DRAQ5™ to stain the nuclei of neurons maintained in culture that were infected with herpes simplex virus 1 and processed for indirect immunofluorescence.  Samples were analyzed by confocal microscopy.

Name: Angela Pearson

Organisation: Institut National de la Recherche Scientifique

"Good product."

PRODUCT:                 DRAQ5

Application Area:       Nuclei staining for microscopy imaging analysis.

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          3.7

Comments: It is easy to use. It can substitute DAPI, but it is the far-red that occupy one channel for other fluorophores. I used it for certain microscope that did not have DAPI channel.

Name: Dandan Liu

Organisation: University of Missouri-Columbia

"Easy to use, great results."

PRODUCT:                 DRAQ5

Application Area:       Cell staining.

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.7

Comments: Product is good, always works, good investment of money.

Name: elizaveta fasler-kan

Organisation: inselspital bern

"Great results and no time consuming staining."

PRODUCT:                 DRAQ5

Application Area:       Analyze morphology of human cells/ analyze the influence of nanomaterials on cells.

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: We noticed a good staining with DRAQ5™ of cell nuclei and repeatability of experiment. We obtained a good signal under the confocal microscopy. The DRAQ5™ dye doesn't bind unspecifically.

Name: Alicja Warowicka

Organisation: NanoBioMedical Centre, UAM

"Worked very nicely, we use it continuously."

PRODUCT:                 DRAQ5

Application Area:       Analysis of cell division.

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: The product is very easy to use and very efficient in its application.  We have used it in the study of mitotic spindle orientation to identify the specific stages of mitosis. The product always worked very reliably in our hands. It could, however, a bit cheaper.

Name: Klaus Ebnet

Organisation: Institute of Medical Biochemistry, ZMBE, University Muenster, Germany 

"we usually use it in routine analysis"

PRODUCT:                 DRAQ5

Application Area:       staining of living cells

Ease of Use:              ⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          3.3

Comments: Very easy product for the staining of cells in FASC analysis.

Name: Knut Biber

Organisation: University Hospital Freiburg 

"Highly recommend it. Works great for ImageStream"

PRODUCT:                 DRAQ5

Application Area:       Stain ovarian cancer cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: DRAQ5 is extremely easy to use for our application: i.e. staining nuclei of cells for high imaging flow cytometry. Reproducible results every time. 

Name: Manos Karteris

Organisation: Brunel University London 

"Great results"

PRODUCT:                 DRAQ5

Application Area:       Analyze nuclei in cellular preparations

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: Very easy to use, have the great advantage to be usable in live cells.

Name: Paolo Raffa

Organisation: Molecular Medicine Department - University of Padova

"Great label for the nucleus"

PRODUCT:                 DRAQ5

Application Area:       Marker for the nucleus

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 is a high quality far red nuclear label that we frequently use in semi-automated confocal microscopy experiments. The dye appears to behave very well--it is easy-to-use, stable, and provides a high quality label for the nucleus of live cells.

Name: Lewis Kraft

Organisation: Harvard University

"Solid results, quite pleased with this product."

PRODUCT:                 DRAQ5

Application Area:       Analyze pore formation in host cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          4.7

Comments: After assay optimization, this product worked quite well for imaging host cells by microscopy during bacterial infection. The product has a long shelf life, provided consistent results, and was not that expensive. The only reason I gave 4 starts for the After Sales Care is because I had no direct interaction with them, but I think our lab tech found the initial instructions with the package confusing. Overall, it is a great product for a specific assay.

Name: Walter Adams

Organisation: Tufts University 

"Superior to propidium iodide."

PRODUCT:                 DRAQ7

Application Area:       cell viability, FACS

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ7, a cell impermeant DNA stain, is great to assess cell viability using FACS: low autofluorescence in the infrared, ease to combine it with other fluorophores in the visible range. Much better than propidium iodide on the last account.

Name: Francois Marceau

Organisation: CHU de Quebec - Université Laval, Canada

"Excellent product, would thoroughly recommend it"

PRODUCT:                 DRAQ5

Application Area:       Fluorescence labelling of cell nuclei

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: Draq5 is reliable and easy to use. It has many advantages over other staining methods. We have used it for years now, and have always been happy with the results. It is now the standard staining methods for many techniques in my lab.

Name: Jonathan Sleeman

Organisation: University of Heidelberg, Germany

"Good product"

PRODUCT:                 DRAQ5

Application Area:       Analyze cell cycle in B cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: Very easy to use, you obtain nice cell cycle graphs.

Name: Carmen Donate

Organisation: Hôpitaux Universitaires de Genève (HUG)

"Perfect Product"

PRODUCT:                 DRAQ5

Application Area:       Flow cytometry - To stain the nucleus of myoblast and myotubes cultures 

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          4.7

Comments: DRAQ5 is easy to use and it gives us a good reproducibility and effectiveness. In my experiments, it was a really helpful product to stain the nucleus of my cells in an easy way. In addition, the quality of DRAQ5 is sufficient high for a broad range of several flow cytometry experiments. I am very pleased with the product and would recommend it to anyone.

Name: Tanhee Sente

Organisation: 1989

"The marker works very well for imaging of cell spreading and growth within and or on the scaffolds"

PRODUCT:                 DRAQ5

Application Area:       Cell adhesion and growth marker

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: It is very easy to use and we obtained very significant results with the product

Name: Hilal Turkoglu Sasmazel

Organisation: Atilim University

"useful product"

PRODUCT:                 CyGEL
Application Area:        Frog embryo research

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments:                 This is an effective product that we find is relatively easy to use.

Name: Tim Mohun

Organisation: The Francis Crick Institute

"Works with 2-photon excitation at 1275 nm."

PRODUCT:                 DRAQ5

Application Area:       DNA staining in 2P microscopy

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: We used it for live DNA-staining in 2Photon microscopy with long wavelengths (1275 nm), so we could use it in parallel with Third Harmonic Generation (THG) detection. Worked as expected. 

Name: Steffen Dietzel

Organisation: University of Munich

"Outstanding results, isn't the cheapest nuclear label, but works well"

PRODUCT:                 DRAQ5

Application Area:       Live cell imaging

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.3

Comments: DRAQ5 works well as a nuclear label and allows us to use a different spectral range for configuring our multi-label assays.  It appears (not 100% sure why) that DRAQ5 has less particulate and non-specific labelling in our microvascular endothelial cells than Hoechst. 

Name: Silas Leavesley

Organisation: University of South Alabama

"Draq5 is an easy and efficient fluorescent Live-Cell Permeant DNA Dye to perform accurate normalization in Cell Western Blotting."

PRODUCT:                 DRAQ5

Application Area:       Cell number normalization of fluorescence signal between wells in cell Western Blotting

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: The product Draq5 is a ready-to-use aqueous formulation really easy to use (simple incubation and washes) and it gives us a good reproducibility between experiment. 

It has a good stability after storage.

Draq5 is a stable fluorescent Live-Cell Permeant DNA Dye which allow us to normalize the fluorescence signal between wells in an in Cell Western Blotting (Odyssey). We followed protein expression (Fluorescence 1) in bone marrow derived macrophages after different cell culture conditions and Draq5 signals (Fluorescence 2) provided us a good linearity for normalization of protein signals well-to-well (ration Florescence1/Fluorescence2).

Name: François CANONNE-HERGAUX

Organisation: INSERM , Intitut de Recherche en Santé Digestive (IRSD), Toulouse, France

"reliable, user-friendly product"

PRODUCT:                 CyGEL
Application Area:        Live cell imaging by confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 We have used CyGEL to immobilize Drosophila pupae for live imaging using a confocal microscope. Embedding of samples in glass bottom dishes is carried out using a fluorescence stereomicroscope. We find CyGEL more convenient than LMP agarose because it saves the hassle to melt agarose and have a heating block or water bath nearby to keep agarose liquefied. The only utensil required is a small container with ice to keep CyGEL in its liquid form. Toxicity was not an issue since samples survived 3D time-lapse imaging for five days (see http://rsos.royalsocietypublishing.org/content/3/2/150517). Overall, we are very satisfied with this product.

Name: Martin Wasser

Organisation: BioImagingMW

"Great product!"

PRODUCT:                 DRAQ5

Application Area:       Analyze proteins in cell and tissue samples 

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: This product gives high quality and reproducible results

Name: Marina Arbi

Organisation: University of Patras

"Great for imaging cell death as well as FACS"

PRODUCT:                 DRAQ7

Application Area:       Small molecule cell death screens 

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: Draq7 offers a great means in which to image cell death in real time. Although the product is commonly used as a FACS marker for cell death, I have found great utility as a way to real time mark cell death using Incucyte. Con is price is slightly higher than competitor products used previously, but Draq7 is still my go to marker for cell death

Name: Richard Crispin

Organisation: University of Edinburgh

“Nice option to visualize nuclei in living cells”

PRODUCT:                  DRAQ5

Application Area:        staining of nuclei in living cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: We achieved reproducible results in a high quality. DRAQ5 was really easy to use. We needed small amounts (1:1000) to make nuclei visible during immunofluorescence analysis. An Incubation for 10 minutes with 1:1000 DRAQ5 in Medium was enough to stain nuclei and it wasn’t indispensable to change/discard medium for microscopy.

Name: Jorge Enrique Duque Escobar 

Organisation: Clinical Pharmacology and Toxicology, Hamburg

"Good results"

PRODUCT:                 DRAQ5

Application Area:       Analyze antigen in adrenocortical tissues 

Ease of Use:              ⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.0

Comments: Easy to use, with high quality of results. By Immunofluorescence analysis we have rapidly analyze the nucleuses of our cells. 

Name: Beatrice Rubin

Organisation: University of Padua

.........................................

Manufacturer's response: Thanks for taking the time to review DRAQ5. We would love to know what would make DRAQ5 score 5’s – that would help direct our product development.

"Very good results with Draq5"

PRODUCT:                 DRAQ5

Application Area:       analyzes of protein endocytosis 

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.0

Comments: Draq5 is easy to use and the staining of the nucleus is bright and specific

Name: Verena Rabiej

Organisation: Institute of Pathobiochemistry, Johannes-Gutenberg University Mainz

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Manufacturer's response: Thank you for the positive response on the performance of the product! I’m sorry that we didn’t meet your after-sales expectations but have no record of receiving an after-sales enquiry. We would love to know what we could do better.

"Good product for staining"

PRODUCT:                 DRAQ5

Application Area:       Nuclear staining 

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: The lab personnel have been requesting this product for the last few years for their research studies.

Name: Diana Chan

Organisation: Houston Methodist Research Institute