"Far red cell nucleus stain"

PRODUCT:                  DRAQ5

Application Area:        Cell colocalization analysis

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐
Average rating:          2.7

Comments: DRAQ5 is generally easy to use. The spectra are in long wavelength that can be paired with many other fluorophores and thus our choice of nucleus dye for confocal imaging. However, the performance is not very stable, sometime it can be very dim and unspecific staining outside the nucleus can be seen. The product (from Thermo Fisher) is very expensive. It will be good if the price can be more reasonable.

Name: Yuning Hong

Organisation: La Trobe University

.........................................

Manufacturer's response: Dear Yuning, thank you for detailed review of DRAQ5. Without seeing examples of the issues you raise it is difficult to give further technical advice. The occasional cytoplasmic staining you describe is often a symptom of understaining or washout due to residual surfactant left over from a prior washing step. Regarding cost, there are a number of other carefully selected suppliers you can choose from and we can advise on the most competitive of these or alternatively order direct from us, the source!

"great results"

PRODUCT:  DRAQ7
Application Area:  Flow cell sorting

Ease of Use:	⭐⭐⭐⭐⭐ (5.0)
After Sales Service:	⭐⭐⭐⭐⭐ (5.0)
Value for Money:	⭐⭐⭐⭐⭐ (5.0)
Average Rating:	5.0

Comments:  This product works very well for flow cell sorting.

Name: Chaoqun Wang
Organisation: UCSF
Review Date: 4 January 2022

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"Compared with other nuclear dyes, this is one of the best, at least, in our hands"

PRODUCT:                  DRAQ5

Application Area:        Budding yeast nuclear staining of live cells observations

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: DRAQ5 is very easy to use. You resuspend cells in PBS, stain cells in dark for 1-3 minutes, then you can image cells directly (no rinsing is needed). The product is kind of expensive, but it is worth it. The dye can be excited by either 488 or 639 nm laser, but emits in far red, so it won't interfere with GFP.

Name: Zhaojie Zhang

Organisation: University of Wyoming

"Great results"

PRODUCT:                 DRAQ5

Application Area:        Cells and Tissue imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We achieved high quality and reproducible data.

Name: Cindrilla Chumduri

Organisation: University of Würzburg

"Excellent reproducible results."

 PRODUCT:                 DRAQ5

Application Area:        Fluorescently paint cell nucleus

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The DRAQ5 reagent is very easy to use. I have used it together with DAPI for counterstaining the cellular nuclei in various cell types like SHSY-5Y, SKN-SH, U2OS and HeLa. The reagent is robust and the staining is perfectly reproducible. I had used the probe in a variety of application like Fluorescent Microscopy, FISH and combined FISH with ICC. The probe lifetime is commendable even after multiple exposures. You can find images with application of DRAQ5 in my publication here - Chanda, K., Jana, N.R. & Mukhopadhyay, D. Receptor tyrosine kinase ROR1 ameliorates Aβ1–42 induced cytoskeletal instability and is regulated by the miR146a-NEAT1 nexus in Alzheimer’s disease. Sci Rep 11, 19254 (2021). https://doi.org/10.1038/s41598-021-98882-0

Name: Kaushik Chanda

Organisation: The Scripps Research Institute, Florida

"Affordable results."

PRODUCT:                 DRAQ5

Application Area:        Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product has worked very well - giving reproducible results.

Name: Sergio Comincini

Organisation: UNIVERSITY OF PAVIA

"very useful stain to interpret microscopy data"

PRODUCT:               CyTRAK Orange

Application Area:      Visualize nuclei and cell cytoplasm by fluorescent confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:            5.0

Comments:                 Easy to use stain, relatively inexpensive, good stain to localize intracellular organelles and nuclei.

Name: Leroy Versteeg

Organisation: Baylor College of Medicine

"Great product!"

PRODUCT:                 DRAQ5

Application Area:        Imaging of engineered tissues

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product is easy to use and gives us a nice consistent nuclear signal in our engineered tissue models. Since it emits in far-red, it's great for multiplexing with common fluorophores that emit in green/blue/red.

Name: Aleksandra Fomina

Organisation: University of Toronto

"It is one of the best probes I have ever worked on!"

PRODUCT:                 DRAQ5

Application Area:        Cell counting in high-content compound screening

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We have run high-content compound screening and used this product for cell counting. It works in every experiment:  the staining is simple, rapid and efficient, the signal is strong, it does not interfere with other probes.

Name: Xiaofeng Jiang

Organisation: Harvard University

"Great results."

PRODUCT:                 DRAQ5

Application Area:        Mark cellular nucleus

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: I acquired high-quality results.

Name: Francesco Bellanti

Organisation: University of Foggia

"A necessary tool for immunocytochemistry."

PRODUCT:                 DRAQ5

Application Area:       Immunocytochemical analysis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: This product is very easy to use and can stain nuclei with high sensitivity.

Name: N/A

Organisation: N/A, Japan

"nice results"

PRODUCT:                 CyTRAK Orange
Application Area:        2-photon live-imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments:                 We used CyTRAK Orange to label nuclei of acute brain slices and performed 2-Photon live-imaging. The labeling is really easy and fast to process. This dye is very efficient to label nuclei in thick tissue (300 µm) and did not show any toxic effect.

Name: Christelle Cadilhac

Organisation: Université de Montpellier

BioStatus Response:  Dear Christelle, thank you for this review of CyTRAK Orange! We believe this is the first example of its use in 2-photon imaging and it was impressive to see detection through the full depth of 300 micron brain slices.

"Easy & quick"

PRODUCT:                  DRAQ5

Application Area:        In Cell Western - protein normalization

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: Had no issues with the DRAQ5 and it produced reliable and repeatable staining of DNA.

Name: Niels Asmussen

Organisation: VCU

"Great results."

PRODUCT:                 DRAQ5

Application Area:        Cell Biology

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It’s a very good product for our application. It is very easy to use.

Name: Emanuela Senatore

Organisation: Università Federico II Napoli

.........................................

Manufacturer's response: Thank you for your opinion Emanuela! It’s great to have this positive feedback!

"Blue cells."

PRODUCT:                 DRAQ5

Application Area:        Identification of single nucleated cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Easy to use, very potent, just add to your cells and their nuclei will turn blue. Easy peasy lemon squeezy.

Name: hector tejeda

Organisation: erasmus mc

.........................................

Manufacturer's response: Thanks Héctor, for this entertaining review of DRAQ5! You make a good point too: it is possible to view DRAQ5-positive cells with transmission light microscopy - the nuclei are indeed deep blue (as well as far-red fluorescent)! It’s a convenient way to test for functionality and sample quality (for example prior to FACS) and might even allow for a new breed of assays.

"The product is of high quality and easy to use. I would recommend it to everyone."

PRODUCT:                 DRAQ5

Application Area:        Cell staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is reliable and works out great. It gives me consistent staining results with high quality. It stains nuclei easily and produces clear images suitable for publication.

Name: Wen Shi

Organisation: University of Nebraska Medical Center

"Great results."

PRODUCT:                  DRAQ5

Application Area:        Live cell nuclear staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Stable and specific signals for live cell nucleus imaging. Very easy to use.

Name: Zhou Zhou

Organisation: Peking University

"The most effective nuclei stain for live cells in our cell type of interest."

PRODUCT:                 DRAQ5

Application Area:        FACS analysis, IF microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: The product was highly effective in helping us identify the nuclei of both live/dead cells. I will be purchasing this product again and believe it to be highly reliable and effective.

Name: Alecia-Jane Twigger

Organisation: University of Cambridge

"Very good."

PRODUCT:                 DRAQ5

Application Area:        Fluorescence imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: I am very satisfied with the product and I often recommend it to other researchers, especially due to its excitation/emission spectrum. In fact, it is very suitable for those who work with autofluorescent scaffolds/biomaterials. It is easy to use, it is a vital dye and marks the cell nucleus well, although sometimes it provides a slightly dirty staining.

Name: Emanuela Jacchetti

Organisation: POLITECNICO DI MILANO

"It is a very good option for labelling cell nuclei."

PRODUCT:                  DRAQ5

Application Area:        Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: This product is very easy to use and always offer reproducible results.

Name: Laura Frago

Organisation: Universidad Autónoma de Madrid 

"Great results!"

PRODUCT:                  DRAQ7

Application Area:        Detect double-strand DNA in tissue samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: In our study, cell-impermeable DNA dye DRAQ7 was injected  to explore potential host DNA release. This reagent helped us to prove our experiment could induce DNA release easily.  We have published our paper!

Name: Yan Cao

Organisation: University of Rhode Island 

"Great product, only a little bit pricy!"

PRODUCT:                  DRAQ5

Application Area:        Analyze endosome position respect to the nucleus in mammalian cells

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐
Average rating:          2.7

Comments: The DRAQ5 is an easy usable marker to stain nucleus. I used it in mammalian cells. It is a really good product, maybe a little expensive.

Name: Giulia Villari

Organisation: university of Turin

.........................................

Manufacturer's response: Dear Giulia, thank you for your valued feedback and independent review. If you have a concern over local pricing we can assist by supplying direct. Also, the small cost of a proven, reliable nuclear counterstain that can be used for 1000-2000 slides should be seen against the significant cost of multiple fluorescently-tagged monoclonal antibodies used alongside it and that its far-red emission enables.

"Our staff prefer DRAQ7 for detecting dead cells using flow cytometry."

PRODUCT:                  DRAQ7

Application Area:        Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We have switched to using DRAQ7 for all of our flow cytometry experiments for the detection of dead cells.

Name: Kate Appleton

Organisation: KIYATEC, Inc. 

"Great results."

PRODUCT:                  DRAQ5

Application Area:        Cell nuclei staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: Good cell staining to characterize the model by means of confocal microscopy.

Name: Valentina O.

Organisation: Italian Institute of Technology

"Great results."

PRODUCT:                  DRAQ5

Application Area:        Characterization of cell therapy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use. Reproducible. Slight improvement would be to have an already diluted form.

Name: Pauline François

Organisation: Assitance Publique Hôpitaux de Marseille AP-HM

.........................................

Manufacturer's response: Dear Pauline, thank you for the generous review and the constructive suggestion. We have produced a diluted presentation of DRAQ5 (at 500 µM rather than 5 mM). The product code is DR05500 and, having reviewed your protocol, would perhaps allow you to skip the pre-dilution step entirely e.g. 4 µl into a final volume of 100 µl would give a final concentration of 20 µM. Perhaps this might assist you better?

"Stable results."

PRODUCT:                  DRAQ5

Application Area:        Enucleation of red blood cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and the procedure is not complicated. We used this stain to evaluate the enucleation rate of cultured red blood cell differentiated from CD34+ cells.

Name: Xin Zhang

Organisation: First affilated hospital of Shantou University Medical College

"Easy-to-use DRAQ5 with excellent results."

PRODUCT:                  DRAQ5

Application Area:        Immunofluorescent analysis of fixed mouse brain tissues

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is an easy-to-use product that is ready straight from the bottle and just have to be diluted to a working concentration. As with almost all dyes and antibodies for immunofluorescence, it has to be tested for the ideal concentration but the product came with suggested working solution concentrations that proved to be very useful as I had to do just a couple of tests to establish the working conditions. The dye is very bright and long-lasting, and the reagent itself lasts for long time at 4 degrees C.

Name: Mitchell Marta

Organisation: NYU Langone Health 

"Great results and easy to be use."

PRODUCT:                  DRAQ5

Application Area:        Immunology

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and labeled my cells very well. I recommend using it in cell assays such as image flow cytometry.

Name: Camila Pontes

Organisation: UNIFESP

"Great results."

PRODUCT:                  DRAQ5

Application Area:        Cell culture

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It is very easy to use. Only takes 15 minutes.

Name: Noemi García Romero

Organisation: Universidad Francisco de Vitoria

"DRAQ5 is my favorite!"

PRODUCT:                  DRAQ5

Application Area:        Microscopy - DNA stain

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Love DRAQ5 for simple and bright nuclear staining of live or fixed cells for confocal microscopy. Minimal crossover in typical filter sets.

Name: Leon Friesen

Organisation: University of Michigan

"Useful fixable DNA stain for assessments of cell membrane integrity."

PRODUCT:                  DRAQ7

Application Area:        Fixable DNA stain

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Good DNA staining. Does not leak into viable cells after brief formaldehyde fixation.

Name: Simon Cleary

Organisation: UCSF

"DRAQ5 is bright and stable"

PRODUCT:                 DRAQ5

Application Area:        nanoparticles and cells interactions

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐
Average rating:           3.7

Comments: I've been using DRAQ5 for some time, I like it very much, it's efficient (bright and quite stable), but I wish it was cheaper.

Name: Marcelo Bispo de Jesus

Organisation: University of Campinas

.........................................

Manufacturer's response: Dear Marcelo, Thank you for taking the time to review DRAQ5. We have no control over end-user prices but it is possible to purchase direct from BioStatus, where we are transparent about our pricing - published on our website in GBP, USD and EUR. However, we will investigate this directly with you to find a better, less expensive way for you and other scientists in Brazil to get the far-red benefits of DRAQ5!

"Easy to use, reliable nuclear stain."

PRODUCT:                 DRAQ5

Application Area:        Nuclear stain for cell counting

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use, reliable nuclear stain. Can be used for the analysis of cell counts on primary human monocytes, monocyte-derived macrophages and dendritic cells. One vial is enough for a long time as it is used in a 1000x dilution.

Name: Szilvia Lukácsi

Organisation: Eötvös Loránd University 

"Great results"

PRODUCT:                 DRAQ5

Application Area:        Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Draq5 is very easy to use and gives reproducible resutls. In each immunostaining that I used Draq5 always worked fine.

Name: Maria Gomez-Casati

Organisation: Universidad de Buenos Aires 

"The innovative approach gave positive results."

PRODUCT:                 DRAQ5

Application Area:        Studying microbial distribution in porous materials

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We have used DRAQ5 to follow the bacterial distribution on wooden material. Our results are cited in (doi:10.3390/antibiotics9050225) and (doi:10.1007/s00107-018-1294-z).

Name: Muhammd tanveer Munir

Organisation: ESB

"Quick and easy!"

PRODUCT:                  DRAQ7

Application Area:        Analyze cell death in immune cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:           4.7

Comments: Easy to use, reproducible and consistent results.

Name: Alexander Wood

Organisation: University of Cambridge

"Decent nuclear staining in far red channel."

 PRODUCT:                 DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: If there is the need to visualize fixed nuclei by confocal microscopy in the far red, then Draq5 is the stain of choice. Of course it is not as bright as DAPI and probably the "gain" and "laser power" should be considerably increased. Also some leakage of staining in cytoplasm could be observed, but still it is very reliable for staining. For the best results, dilution 1:500 in 0.1M PBS (alternatively with 0.3% Triton X) should be used.

Name: Juraj Sevc

Organisation: Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Slovak Repub

"Simple and robust results."

PRODUCT:                  DRAQ7

Application Area:        Live cell microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: Simple and easy to use. Little to no background fluorescence (wide-field microscopy, LED illumination). Very clear and strong signal upon cell death.

Name: Joe Giovinazzo

Organisation: CU Anschutz

"Great consistent staining in fixed brain sections."

PRODUCT:                  DRAQ7

Application Area:        Immunofluorescence: PFA fixed brain tissue sections.  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Product works consistently well for immunofluorescence on PFA fixed brain sections. I used a dilution of 1:1000 in PBS 1x for 15 minutes and it works flawlessly every time.

Name: Robert Lewis

Organisation: University of California, Irvine School of Medicine

"Easy-to-use product."

PRODUCT:                  DRAQ5

Application Area:        Flow cytometry live/dead staining observations

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and provides clear live/dead staining of multiple sample types.

Name: Sharon Sagnella

Organisation: EnGeneIC Pty Ltd