"DRAQ5 is my favorite!"

PRODUCT:                  DRAQ5

Application Area:        Microscopy - DNA stain

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Love DRAQ5 for simple and bright nuclear staining of live or fixed cells for confocal microscopy. Minimal crossover in typical filter sets.

Name: Leon Friesen

Organisation: University of Michigan

"Useful fixable DNA stain for assessments of cell membrane integrity."

PRODUCT:                  DRAQ7

Application Area:        Fixable DNA stain

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Good DNA staining. Does not leak into viable cells after brief formaldehyde fixation.

Name: Simon Cleary

Organisation: UCSF

"DRAQ5 is bright and stable"

PRODUCT:                 DRAQ5

Application Area:        nanoparticles and cells interactions

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐
Average rating:           3.7

Comments: I've been using DRAQ5 for some time, I like it very much, it's efficient (bright and quite stable), but I wish it was cheaper.

Name: Marcelo Bispo de Jesus

Organisation: University of Campinas

.........................................

Manufacturer's response: Dear Marcelo, Thank you for taking the time to review DRAQ5. We have no control over end-user prices but it is possible to purchase direct from BioStatus, where we are transparent about our pricing - published on our website in GBP, USD and EUR. However, we will investigate this directly with you to find a better, less expensive way for you and other scientists in Brazil to get the far-red benefits of DRAQ5!

"Easy to use, reliable nuclear stain."

PRODUCT:                 DRAQ5

Application Area:        Nuclear stain for cell counting

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use, reliable nuclear stain. Can be used for the analysis of cell counts on primary human monocytes, monocyte-derived macrophages and dendritic cells. One vial is enough for a long time as it is used in a 1000x dilution.

Name: Szilvia Lukácsi

Organisation: Eötvös Loránd University 

"Great results"

PRODUCT:                 DRAQ5

Application Area:        Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Draq5 is very easy to use and gives reproducible resutls. In each immunostaining that I used Draq5 always worked fine.

Name: Maria Gomez-Casati

Organisation: Universidad de Buenos Aires 

"The innovative approach gave positive results."

PRODUCT:                 DRAQ5

Application Area:        Studying microbial distribution in porous materials

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We have used DRAQ5 to follow the bacterial distribution on wooden material. Our results are cited in (doi:10.3390/antibiotics9050225) and (doi:10.1007/s00107-018-1294-z).

Name: Muhammd tanveer Munir

Organisation: ESB

"Quick and easy!"

PRODUCT:                  DRAQ7

Application Area:        Analyze cell death in immune cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:           4.7

Comments: Easy to use, reproducible and consistent results.

Name: Alexander Wood

Organisation: University of Cambridge

"Decent nuclear staining in far red channel."

 PRODUCT:                 DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: If there is the need to visualize fixed nuclei by confocal microscopy in the far red, then Draq5 is the stain of choice. Of course it is not as bright as DAPI and probably the "gain" and "laser power" should be considerably increased. Also some leakage of staining in cytoplasm could be observed, but still it is very reliable for staining. For the best results, dilution 1:500 in 0.1M PBS (alternatively with 0.3% Triton X) should be used.

Name: Juraj Sevc

Organisation: Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Slovak Repub

"Simple and robust results."

PRODUCT:                  DRAQ7

Application Area:        Live cell microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: Simple and easy to use. Little to no background fluorescence (wide-field microscopy, LED illumination). Very clear and strong signal upon cell death.

Name: Joe Giovinazzo

Organisation: CU Anschutz

"Great consistent staining in fixed brain sections."

PRODUCT:                  DRAQ7

Application Area:        Immunofluorescence: PFA fixed brain tissue sections.  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Product works consistently well for immunofluorescence on PFA fixed brain sections. I used a dilution of 1:1000 in PBS 1x for 15 minutes and it works flawlessly every time.

Name: Robert Lewis

Organisation: University of California, Irvine School of Medicine

"Easy-to-use product."

PRODUCT:                  DRAQ5

Application Area:        Flow cytometry live/dead staining observations

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and provides clear live/dead staining of multiple sample types.

Name: Sharon Sagnella

Organisation: EnGeneIC Pty Ltd

"Good results; recommended in the current histological practice."

PRODUCT:                  DRAQ5

Application Area:        Neuropathology

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Good nuclear marker for single- and double-labelling immunofluorescence and confocal microscopy on frozen and paraffin-embedded sections. Staining disappears in sections pre-treatment with formic acid (as in prion diseases). Sometimes, non-specific staining of other structures.

Name: Isidro Ferrer

Organisation: University of Barcelona, Bellvitge University Hospital, IDIBELL, CIBERNED

.........................................

Manufacturer's response: Thank you very much for the valuable feedback and product review on DRAQ5. The observation about the use of formic acid is useful to know. Perhaps it may be worthwhile to attempt staining with DRAQ5 as the final step after all other steps are complete, including the formic acid treatment. It is unusual to see staining of other structures outside of the nucleus with DRAQ5 but may be a consequence of the treatments of the sample. DRAQ5 does have, like all cell permeant drug-like small molecules, a propensity for binding to lipophilic structures in addition to the specific target, in its case dsDNA, but this is usually only seen in cases of understaining, paradoxically. We are always ready to answer these questions through our tech support service as they occur in real-time in the bench experiments.

"Great results."

PRODUCT:                  DRAQ7

Application Area:        Analyze apoptosis  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Solved an issue in our experiment that PI has overlapping excitation and emission wavelength with high concentration Adriamycin. We got desirable results after using DRAQ7 to label late-apoptotic cells.

Name: Zhi Chen

Organisation: Shimane University, Medical Faculty (Japan)

"Nucleus stain doing its job"

 PRODUCT:                  DRAQ5

Application Area:        Cell imaging co-localization analysis

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.3

Comments: We used DRAQ5 because of its far-red fluorescence and suitability for live/fixed-cell imaging. In general, it suits our needs as we can't find a commercial alternative out there. It is easy to use, but sometimes the fluorescence can be very dim and needs some adjustment to make it visible. We bought it from ThermoFisher/Invitrogen so it is quite expensive.

Name: Yuning Hong

Organisation: La Trobe University

"Best results."

PRODUCT:                  DRAQ5

Application Area:        Cell culture studies

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We have been using the product for a while and will now use it for most of our biological studies. The reproducibility was good. I have already recommended the product to my colleagues. Perfect value for money.

Name: Anshuman Jakhmola

Organisation: Italian institute of Technology Napoli (CRIB)

"Best for our experiments."

PRODUCT:                  DRAQ7

Application Area:        Imaging cells in real time  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This dye has changed the way we can perform experiments so that they can be maintained for longer without losing cell viability. Together with Hoechst, it means we can estimate the proportion of dead cells in an entire population quickly, easily and over several time points.

Name: Natasha Lewis

Organisation: Enplas

"Easy stain."

PRODUCT:                  DRAQ5

Application Area:        Evaluation of erythroid cell enucleation

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 is easy to use and gives reproducible results. It can be added to the cells at the last moment notably after performing antibody staining and does not require washing the cells prior analysis.

Name: Emmanuel Olivier

Organisation: Albert Einstein College of Medicine

"Avoid when studying DNA replication /repair /transcription /organization in cellulo."

PRODUCT:                  DRAQ5

Application Area:        Supravital DNA fluorescent dye

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐
Average rating:           3.0

Comments: DRAQ5 is fine unless you want to study DNA replication, repair, transcription, etc in living cells. Why? Because, like other DNA intercalators, DRAQ5 perturbs the DNA macromolecule and will evict histones and a variety of DNA binding proteins.

Name: PO MARI

Organisation: CNRS

.........................................

Manufacturer's response: Dear Pierre-Olivier, Thank you for your review of DRAQ5. It is indeed a well-understood phenomenon that all cell-permeant DNA-binding probes will have some impact on cell behaviour. Accordingly, we alert users to this on the front of the product’s technical datasheet. Nonetheless, DRAQ5 has been widely used for live-cell end-point assays and as a means of identifying the nucleated cells in flow cytometry experiments on complex sample matrices such as bone marrow, besides its popularity as a standard IF counterstain.

"excellent results"

PRODUCT:                 CyGEL
Application Area:        Leishmania mexicana live cell imaging

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments:                 CyGel was very convenient to use and I would recommend it to everyone working on leishmania.

Name: Jaspreeet Grewal

Organisation: University of Warwick

"Great results! "

PRODUCT:                  DRAQ5

Application Area:        Nuclei staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Excellent and reliable staining of the nuclei! The only drawback is that sometimes it stains more than just the nuclei. It helped with the cell counting program.

Name: Rejhana Kolasinac

Organisation: Research Center Juelich GmbH

.........................................

Manufacturer's response: Thank you for your review of DRAQ5. DRAQ5 does have a useful weak secondary staining of the cytoplasm which HCS labs use to segment the cell envelope, by increasing the gain. An unwanted inversion staining (“hollow” nuclei) is a consequence of too low a concentration of DRAQ5. We strongly recommend 5 uM for fixed cells and 5-10 uM for live cells. We would be very happy to discuss this and troubleshoot the issue to try to resolve it.

"Standard nuclei/DNA counterstain in our lab for more than 15 years!"

PRODUCT:                  DRAQ5

Application Area:        DNA counterstain for immunofluorescence studies

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Super easy to use, just add it to your secondary antibody solution in immunofluorescence studies (cells and tissues), and you get reproducible stainings of your nuclei with excitation/emission wavelenghts that do not interfere with other common flurophores used in multiple immunofluorescence stainings.

Name: Maren Rehders

Organisation: Jacobs University Bremen

"Very good."

PRODUCT:                  DRAQ5

Application Area:        HIV infection

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: The product is reliable, efficient and easy to use. It's results were very good.

Name: Ran Taube

Organisation: BGU 

"DRAQ7 is now implemented in our standard SVF cells staining protocol. "

 PRODUCT:                  DRAQ7

Application Area:        Detection of stromal vascular (SVF) cells isolated from lipoaspirate and microfragmented lipoaspirat  

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ7 is a very stable and easy-to-use dye. We carefully titrated dye using fix/perm approach after the staining for 7 surface markers. After optimization of the staining protocol we achieved strong signal with an easy discrimination of nucleated cell from the cellular debris and fat particles. Samples were analysed on Navios flow cytometer.

Name: Denis Polancec

Organisation: Srebrnjak Children's Hospital

"excellent for mounting live cells for microscopy"

PRODUCT:                 CyGEL
Application Area:        Mounting bacteria for live cell confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 Cygel has been a great addition to my microscopy toolbox! It enables me to carry out imaging of motile bacteria without needing to fix them. Very easy to use, and seems to go a long way. Can highly recommend.

Name: Rhia Stone

Organisation: Institute for Molecular Bioscience, University of Queensland

"Great result"

 PRODUCT:                 DRAQ7

Application Area:        Analyze cell viability by FACS and confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: I found DRAQ7 with a very bright color specifically by using it in imaging. It is also very easy to use, just a drop and no long incubation is needed.

Name: Aida Aida

Organisation: Uppsala University

"Great results, very useful for my experiments."

 PRODUCT:                 DRAQ5

Application Area:        Stain bacteria

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: This product was effective for my experiments. I successefully stain my bacteria for my live/dead assays. I obtained good results and I consider that this product is easy to use.

Name: Bruna Costa

Organisation: Institute for Research and Innovation in Health Sciences (i3S)

"Good and reproducible results."

PRODUCT:                 DRAQ5

Application Area:        Fluorescence microscopy

Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.0

Comments: The product is very easy to use, not too expensive. it does not need specific after sale care. The product allows for reproducible results.

Name: Marco Folini

Organisation: Fondazione IRCCS Istituto Nazionale dei Tumori di Milano

"The dye worked great in our experiment."

 PRODUCT:                  DRAQ5

Application Area:        Drug discovery and screening

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The Draq5 dye worked excellently in our assay. We used it for an image based assay for screening our drug candudates.

Name: Farnaz Zahedifard

Organisation: Pasteur Institute

"Great results."

 PRODUCT:                 DRAQ7

Application Area:        Cell death

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Very effective and easy to use. Been using the product for a very long time to identify cell death in treated tumor specimen.

Name: Kayla Simeone

Organisation: CRCHUM

"It is a good product."

PRODUCT:                  DRAQ5

Application Area:        This product has been used in flow cytometry assays

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: This product has been used in flow cytometry assays. This product is characterized by easy operation. It suffices to follow the manufacturer's guidelines. The results are reproducible. The product stands out further on account of its functionality and quality/price ratio.

Name: Eva Rivero Buceta

Organisation: Instituto de Tecnología Química

"Very easy to use and works well."

PRODUCT:                  DRAQ5

Application Area:        Cell nucleus staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: Very easy to use and works well. Gives reliable and reproducible results.

Name: Rodriguez Alcazar Juan

Organisation: Institute of Innate Immunity - BMZ - University Hospital of Bonn

"Easy to use and yields consistent results."

 PRODUCT:                  DRAQ5

Application Area:        Cellular viability staining for flow cytometry. Used in concert with propidium iodide to differentia

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product allowed us to effectively differentiate live and dead cells and debris when used in concert with propidium iodide for flow cytometric analysis. DRAQ5 can be diluted directly in FACSFlow when resuspending cells and offers a short incubation time. We were able to use this DRAQ5 when analyzing viability in several cell treatment groups and the results obtained matched the expected results.

Name: Andrew Reichard

Organisation: Cleveland Clinic Lerner Research Institute

"Good, reliable, easy to use product."

PRODUCT:                  DRAQ5

Application Area:        In Cell Western analysis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:          4.3

Comments: DRAQ5 is a versatile and easy to use DNA stain. We use it for high/medium throughput gene perturbation screens in mammalian cells.

Name: Klaas Mulder

Organisation: Radboud University

"Good results."

PRODUCT:                  DRAQ5

Application Area:        Analyze efficacy of novel compounds

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
 Average rating:           4.0

Comments: The product was easy to use and the results were reproducible. I would recommend the product.

Name: Aya Hefnawy

Organisation: Institute of tropical medicine

"Can't live without it"

 PRODUCT:                  DRAQ5

Application Area:        Analyze Nanoparticles-Cell interaction

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.0

Comments: It's a very good product! I use it for fluorescence and confocal microscopy and i really like it

Name: Allan Radaic

Organisation: UNICAMP

.........................................

Manufacturer's response: Dear Allan, thank you for your positive review of DRAQ5. We are keen to understand how we can improve our after-sales service and would appreciate your feedback

"Consistent results, easy to use, what's not to love?"

PRODUCT:                  DRAQ5

Application Area:        Use as measure of cell viability and to determine cell numbers

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:           5.0

Comments: The DRAQ5 is essential for our In-Cell ELISA assays to measure the cell numbers and viability. It is super easy to use and lasts extremely long. It consistently delivers great results and we have never run into any problems with this reagent.

Name: Marcus Ilg

Organisation: Anglia Ruskin University

"Best choice for our application"

PRODUCT:                 DRAQ7

Application Area:       Three-dimensional live-cell imaging on implant

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.7

Comments: We used DRAQ7 to monitor the cell death along implants over time. For this purpose we required a non-toxic and stable fluorescent dye to detect dead cells. DRAQ7 met all necessary requirements and worked also in the hydrogel. It was very easy to use with immediate results without optimization period.  Some other advantages are that it works without UV light and is non-toxic to cells over time.

Name: Alexandra Ingendoh-Tsakmakidis

Organisation: Hannover Medical School

"Great results, I would recommend it."

PRODUCT:                 DRAQ5

Application Area:       DNA marker on mouse cerebellar slices

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.3

Comments: It's an easy-to-use product of high quality. The results are good and reproducible.

Name: Laura Trobiani

Organisation: Sapienza University of Rome

"Very good results, absolutely worth trying."

PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence on fixed brain tissue

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.7

Comments: This product is very easy to use and gives excellent staining, highly reproducible among distinct experiments. The results are comparable to the commonly used DAPI. Very good results also after antigen retrieval protocols. Absolutely worth trying.

Name: Valentina Cerrato

Organisation: university of Turin

"Beautiful microscopy images."

PRODUCT:                 DRAQ7

Application Area:       Labeling nucleous in cleared hidrogel-brain tissue (CLARITY)

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.3

Comments: DRAQ7 has been used in Glioblastoma bystander cell therapy: improvements in treatment and insights into the therapy mechanisms.  Is very easy to use.  We got beautiful microscopy images you could find in our paper.

Name: Cristina Garrido

Organisation: Institute of Advanced Chemistry of Catalonia