"Must have tracer "

PRODUCT:                  DRAQ5

Application Area:        nuclear imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Easy-to-use nuclear marker when other fluorescence emissions than blue (DAPI, Hoechst) are required.

Name: Romain Duval

Organisation: Institute fo Research and Development

"Good reproducible results, more comfortable and suitable than DAPI staining"

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining for indirect immunofluorescence experiments

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 was a very useful and easy to use tool for nuclear staining. In my immunofluorescence experiments I need to stain the nuclear fraction of the cells to have appropriate controls and DRAQ5 gave me this opportunity. Moreover, since the detection is at 633 nm laser range, I can use it instead of DAPI and use 405 nm channel for the detection of other dyes, such as Alexa Fluor 405

Name: Antons Martincuks

Organisation: RWTH Aachen University

"Easy to use, reliable, gives consistent results."

PRODUCT:                  DRAQ5

Application Area:        To stain nuclei in fixed cell culture samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It is very easy to use, gives reproducible results, excellent product!  Good value for the price too. Thank you!

Name: Ibolya Andras

Organisation: University of Miami

"GREAT RESULTS"

PRODUCT:                  DRAQ5

Application Area:        LIVE CELL IMAGING MICROSCOPY

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Very easy use and great results for cell live imaging microscopy

Name: José A Sánchez Alcázar

Organisation: Universidad Pablo de Olavide

"Very useful tool, easy to use and it guarantees good results!"

PRODUCT:                  DRAQ5

Application Area:        Analyze the cytotoxicity of compounds in live cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: It is a very easy to use product. This provides high quality results and images working with live cells.

Name: Ananda Rodilla

Organisation: University of Barcelona

"Great product."

PRODUCT:                  DRAQ5

Application Area:        Identification of nucleated cells by flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: Chose DRAQ5 because of its far red emission and near compensation free properties on the system I was using.  The ability to get total nucleated for gating in log space and then DNA index with careful use in a slightly different red channel using linear is a nice plus.

Name: Tony Ward

Organisation: GPB Scientific

"extremely effective and easy to use as Drop & Go"

PRODUCT:                 DRAQ7 DROP & GO
Application Area:        Cell sorting and Cell viability assays

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 For many years, my core facility users in general and I, personally, have been using several BioStatus products, mainly DRAQ5 and DRAQ7. I have always found them effective and extremely easy to use. DRAQ7 has shown for many years to be a fantastic option for the detection and exclusion of dead cells. Quite recently, we have tested and used DRAQ7 DROP & GO for the detection and exclusion of dead cells from a very heterogeneous sample for isolating photoreceptors from adult zebrafish retinae for downstream RT-PCR. It was as easy as described in the name of the product "drop and go". Dead cells were clearly differentiated from the alive ones as can be easily observed in our recently published paper Glaviano et al. "A method for isolation of cone photoreceptors from adult zebrafish retinae" BMC Neuroscience (2016) 17: 71 (DOI 10.1186/s12868-016-0307-2)

Name: Alfonso Blanco

Organisation: University College Dublin

"Very good and reliable reagent."

PRODUCT:                 DRAQ5

Application Area:        Immunocytochemistry, immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use and cost effective. Great alternative to traditional DAPI in immunocyto-and histochemistry.

Name: Yulia Kiyan

Organisation: Hannover Medical School

"Best nucleic acid stain"

PRODUCT:                  DRAQ5

Application Area:        Staining nuclei in 3D tissue constructs (tissue engineering application)

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Product (DRAQ5) nucleic acid stain works exceptionally well, and keeps common microscope filter channels open since it's a far red stain. Little to no background, easy to use and store. Penetrates deep into thick tissues so you don't observe staining concentration gradients as with other nuclear stains. Perfect for use when imaging 3D tissue constructs with confocal - but also works well for 2D monolayer applications where UV light isn't an option (ex: PMMA microfluidic devices which block UV).

Name: Darren Rodenhizer

Organisation: University of Toronto

"Very useful for in vivo staining, easy to handle, high-quality"

PRODUCT:                  DRAQ5

Application Area:        Staining living mouse embryonic fibroblast nuclei

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: If you are looking for a dye for in vivo staining, DRAQ5 is the best. Even with very little amount of a stock of the product, like 1ul, you can observe bright nuclei immediately. Staining genomic DNA specifically you could capture where DNAs are distributed in nuclei. After decorating your nuclei with it, cells are still happy living healthy. I will keep using the product for my future projects.

Name: Wonki Cho

Organisation: MIT

"Perfect for us."

PRODUCT:                  DRAQ5

Application Area:        Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: Easy substitute for DAPI (we had no DAPI laser available).

Name: Elisabeth Naschberger

Organisation: University Medical Center Erlangen

"Great results, easy to apply."

PRODUCT:                  DRAQ5

Application Area:        Analyze toxicity, analyze the process of apoptosis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: High quality, easy to use, without non-specific staining, good efficiency.

Name: Alicja Warowicka

Organisation: Adam Mickiewicz University, NanoBioMedical Centre

"DRAQ5 is the perfect nuclear stain if no UV-laser is available"

PRODUCT:                  DRAQ5

Application Area:        Nucleus staining in confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: I like that we can stain and visualize nuclei without the need for a UV-laser. The use is very simple, and the sensitivity is very good. We can also differentiate between DRAQ5 and far red probes so we can image several probes in parallel. I also like the fact that the company cares about publications with their products.

Name: Olivia Merkel

Organisation: Wayne State University

"Good Product. Easy to use."

PRODUCT:                  DRAQ5

Application Area:        Staining of Mouse ESC

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Its very easy to use, straight from the fridge without hassle and its reliable.

Name: Hiran Perera

Organisation: UT Health Science Center at Houston 

"We achieved great and specific nuclear staining with this product"

PRODUCT:                  DRAQ5

Application Area:       Immunofluorescence Microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product is very effective. It is also very potent and has high efficacy when used in amounts under the recommended concentration.  Also, the fact that it can be used in live  cells is a huge benefit.

Name: Courtney Copeland

Organisation: Vanderbilt Uiversity 

"Easy to use DNA stain for debris discrimination."

PRODUCT:                 DRAQ5

Application Area:       Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We use DRAQ5 for debris discrimination in CD34+ cell enumeration tests of blood and harvest samples using lyse no wash method. It works very well combined with CD34 PE and CD45 FITC.

Name: Pal Jakso

Organisation: University of Pecs, Dept. of Pathology  

"Great results"

PRODUCT:                 DRAQ5

Application Area:       Cell culturing for evaluation of biocompatibility

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: Easy to use, fast shipping, reasonable price, good results and good quality

Name: Amirkianoosh Kiani

Organisation: University of New Brunswick

"excellent product, affordable, easy to use and gave great results"

PRODUCT:                 CyGEL
Application Area:        Imaging non-adherent leukemia cell line for biosensor application.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 We used CyGel for imaging a K562 cell line to verify that our fluorophore-tagged biosensor is internalized by the cells. The product was very easy to use and worked according to the manufacturer’s description. There was no problem with CyGel interfering with our fluorescent signal using a standard fluorescent microscope for cell imaging. We think it was a very reasonable price for the ease of use and results we obtained. While we did not require follow up service, it was there for our assistance as needed.

Name: Steven Ouellette

Organisation: KinaSense LLC

"DRAQ7 is an easy to use viability dye that allows monitoring of cell death over time."

PRODUCT:                 DRAQ7

Application Area:       Cell viability

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment.  

Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes.  We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible.

Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation.

Name: Daphné Laubreton

Organisation: INRA

"Overall very satisfied with the product and will recommend to all users"

PRODUCT:                 DRAQ5

Application Area:        Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product works great for our applications such as confocal microscopy. We rate this product the best among all the manufacturers. Overall we will order and use this product as long as the prices are kept competitive or we have more superior advantage.

Name: Arun Iyer

Organisation: Wayne State University

"Good product."

PRODUCT:                 DRAQ5

Application Area:        Analyze CTC from blood.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to stain. It was used for stain the nucleus of cells for confocal microscopy.

Name: Xueqing Xia

Organisation: UT MD Anderson Cancer Center

"Great nuclear dye!"

PRODUCT:                 DRAQ5

Application Area:        Immunofluorescence

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Results are quite reproducible and DRAQ5 is easy to use.

Name: Swati Pradhan-Bhatt

Organisation: Helen F. Graham Cancer Center & Research Institute

"This product performed reliably and consistently!"

PRODUCT:                  DRAQ5

Application Area:       Positive control antibody

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: We used DRAQ5 as a nuclear stain to normalize for well-to-well variations in cell density.  This was done in an On-Cell western looking at expression of CB1 surface receptor.

This product was consistent in staining and was an excellent reference stain.

Name: Sandra Leone-Kabler

Organisation: Wake Forest University Health Sciences 

"Great results, highly recommend it."

PRODUCT:                  DRAQ5

Application Area:       Staining of nuclei in live plant cells for visualization of mitosis

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: I used this product to stain nuclei in living cultured plant cells for confocal imaging of mitotic events. I found the dye easy to use and the staining was very quick. The fluorescence was strong and very stable.

Name: Katja Graumann

Organisation: Oxford Brookes University

"Great dye for live or fixed cell imaging."

PRODUCT:                  DRAQ5

Application Area:       Cell segmentation

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: Great dye for live or fixed cell imaging. Especially useful for nuclear and whole cell segmentation.

Name: Caitlin Mills

Organisation: Harvard Medical School

"I achieved reproducible results in imaging and rescuing zebrafish larva using CyGel"

PRODUCT:                 CyGEL
Application Area:        Live Imaging of Zebrafish Larva

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 Imaging mitochondrial dynamics requires that my sample be completely stationary and alive! CyGel allowed me to stabilize zebrafish larva and obtain time-lapse images of mitochondria. I was able to rescue the fish afterwards, so that fish could be imaged on multiple days if necessary.

Name: April Dukes

Organisation: University of Pittsburgh

"Great results. Would recommend to the entire community"

PRODUCT:                  DRAQ5

Application Area:       Confocal imaging of parasites

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Excellent product. I had earlier used Giemsa for staining the parasite in macrophages. I work on a protozoan parasite and once I worked with DRAQ5 phew I got my paper accepted in Autophagy. Would recommend to all.

Name: Rentala Madhubala

Organisation: Jawaharlal Nehru University, New Delhi, India

"DRAQ5 and Retina"

PRODUCT:                 DRAQ5

Application Area:       Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:           5.0

Comments: This is an excellent easy to use marker of cell bodies, especially in retina and brain

Name: donld fox

Organisation: university of houston

"Nice images with confocal laser scanning microscopy"

PRODUCT:                 DRAQ5

Application Area:       Tumor cell lines

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: Worked with diluted stock solution in PBS (better handling and accuracy). The applied incubation time, temperature and concentration were important for good reproducibility. On permeabilized cells the nucleus had better signal and stained clearly.  On the non-permeabilized cells, the cytosol was stained also.

Name: Jozsef Muranyi

Organisation: MTA-TKI 

"Great data achieved, images clear"

PRODUCT:                 DRAQ5

Application Area:       Trafficking in cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: Very effective , very ease of use and one bottle can provide thousands data...

Name: Che Azurahanim Che Abdullah

Organisation: Universiti Putra Malaysia

"Great results and easy use."

PRODUCT:                 DRAQ5

Application Area:       See different nuclei cells types

Ease of Use:              ⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.0

Comments: The react is easy to use and very effective.  We can use it for different cells types and in different conditions.

Name: Marimar Encabo

Organisation: University of Zaragoza 

"Recommended"

PRODUCT:                 DRAQ5

Application Area:       Staining Nucleic Acids

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.7

Comments: We used DRAQ5 for staining of nuclear DNA - and realized that it also nicely stains RNA in Dictyostelium cells, which, for our purpose, was beneficial.

Name: Christian Hammann

Organisation: Jacobs University 

"DRAQ5 is vital to analyze each sample I process"

PRODUCT:                 DRAQ5

Application Area:       Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          4.0

Comments: I use DRAQ5 in order to separate living cells of dead cells in a suspension of brain tumor cells and to calculate the cellular DNA content. I use 3 ul of DRAQ5 3 minutes before the acquisition, therefore I spend several vials of DRAQ5 in a short time and it is an expensive dye.

Name: María González-Tablas

Organisation: Center for Cancer Research 

"Great result, applicable in all of our research"

PRODUCT:                 DRAQ5

Application Area:       Analyse dystrophin in tissue sample

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: High quality, reproducible, easy to use, high intensity

Name: Laetitia GUEVEL

Organisation: UMR703 PAnTher 

"Good nuclear stain"

PRODUCT:                 DRAQ5

Application Area:       Separate two populations based on DRAQ5+/-

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐

 Average rating:          3.7

Comments: Good alternative to Hoechst, DAPI for multiplexing. Stained cells must be washed multiple times to avoid dye leakage into unstained cells.

Name: J Wu

Organisation: MGH

"Good results, bio-safe, easy to use, reliable, helped in visualization of nuclei in live HeLa cells and calculation of Nuclear vs Cytoplasmic ratio of LC3 using MATLAB."

PRODUCT:                 DRAQ5

Application Area:       Nuclei visualization in live HeLa cells

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 was used to visualize nuclei in live HeLa cells using confocal fluorescence microscope LSM 510. This product is very stable and convenient to use. The protocol involving dilution and staining is very simple and easy to follow. The dye is not toxic to HeLa cells and bio-safe. The dye did not interfered with subsequent downstream biochemical analysis e.g. western blot and protein band detection by LI-COR.  DRAQ5 uses far red wavelength, it does not involve the risks associated with UV light as associated with some other nuclear staining dyes in market. Also it was advantageous to us since nuclei visualization was the most essential part of our experiments and DRAQ5 imaging was compatible with our LSM 510.

Name: pallavi manral

Organisation: Vanderbilt University

PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence - nuclei staining

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: DRAQ5 is easy to use, gives reproducible results. Excellent product - good replacement of DAPI.

Name: Przemyslaw Juszczyński

Organisation: IHiT

"Bright signal. More consistent and lower spillover than DAPI."

PRODUCT:                 DRAQ5

Application Area:       Nuclear marker in localization studies (ImageStream), Tissue ImmunoFluorescence

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.3

Comments: We used DRAQ5 as nuclear staining in Flow Cytometry and ImageStream analysis to track the subcellular localization of different proteins. It works well with different detectors and offers a nice alternative to DAPI, leaving UV fluorophores available for other markers. 

This product is also very convenient in tissue immunofluorescence as a cellular marker (nuclei). It allows easy characterization of immune infiltrates in diverse tissues with better and more consistent signal than DAPI.

Name: Gerard Hernandez Mir

Organisation: University of Pittsburgh

"Perfect for nuclear staining!"

PRODUCT:                 DRAQ5

Application Area:       Molecular mechanisms of cell signaling and nuclear/cytoplasmic localization

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐

 Average rating:          4.7

Comments: I have been using this product for past 6 years and it is reliable, perfect and very easy to use.

Name: Yuvaraj Sambandam

Organisation: Medical Universtiy of South Carolina

"Great and reproducible results, I recommend the use of DRAQ5."

PRODUCT:                 DRAQ5

Application Area:       Staining of DNA in rat leukocytes

Ease of Use:              ⭐⭐⭐⭐⭐

After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐

 Average rating:          5.0

Comments: We used DRAQ5 staining for the validation of FSC/SSC dot plot based identification of rat leukocytes from lysed whole blood sample. The use of DRAQ5 is easy, it stained the nuclei instantly. We used DRAQ5 several times and achieved always similar staining intensities, indicating good reproducibility.

Name: Kornelia Szebenyi

Organisation: MTA TTK, Hungarian Academy of Sciences