"Great product! It is absolutely vital for our lab, no nice photos without DRAQ5!"

PRODUCT:                  DRAQ5

Application Area:        Analyze cell culture metabolites - confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product is very easy to use for microscopy, in few minutes your sample is colored.

I will recommend to researchers to use it if you have laser or filters for it.

It is reliable and results are reproducible.

We perform studies on melanoma and retina cell culture, exposed to different stress conditions and we follow their morphology after treatment.

Name: Dumitrita Rugina

Organisation: University of Agricultural Sciences and Veterinary Medicine

"great images and can use in far red region along with green fluorescent fluorophores"

PRODUCT:                  DRAQ5

Application Area:        To view the nuclei for imaging in confocal microscopy in the far-red region

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: easy to use and high reproducibility and can image immediately.

Name: Anita chalasani

Organisation: Wayne State University

"Great results"

PRODUCT:                  DRAQ5

Application Area:        Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 is a good staining to see nucleus in liver cells and tissues. It is efficient in both  paraffin-embedded and OCT-frozen tissue sections. It is easy to use and the results are excellent.

Name: Anna Alisi

Organisation: Bambino Gesù Children's Hospital and Research Institute

"An excellent product"

PRODUCT:                  DRAQ5

Application Area:        Visualize nuclei using confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We used DRAQ5 to label the nuclei of live adult rat ventricular myocytes (see Weeks et. al. β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. Journal of the American Heart Association. 2017 6(4)). We labelled the cells with 1 uM DRAQ5 for 10 minutes prior to imaging, which was lower than the recommended concentration, but it fluoresced nicely.

Name: Kate Weeks

Organisation: Baker Heart and Diabetes Institute

"Great results"

PRODUCT:                  DRAQ5

Application Area:        To analyze nuclei in the cultured isolated bovine chondrocytes

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.0

Comments: The dye was highly effective as even at very diluted concentrations produced excellent results. The confocal images obtained from these chondrocytes whose nuclei were labelled with DRAQ5 produced high quality and reproducible results. I had used this dye to count the number of cells in clusters because in my culture experiments the cell membranes were frequently touching and therefore thresholding of individual cells was impossible. In order to count number of cells in clusters I counted number of nuclei and DRAQ5 gave me highly reliable results as I could count the number of nuclei in 3D by using Volocity (a 3D image analysis software). It is quite an expensive dye but as the very diluted concentrations are very effective therefore, justifies the cost. Moreover, it was easy to use and highly penetrable and required very little time to label nuclei efficiently.

Name: Asima Karim

Organisation: The University of Edinburgh

"Nice alternative to DAPI."

PRODUCT:                  DRAQ5

Application Area:        analyse transplanted tissue

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.7

Comments: Fairly easy to use. Need to be made clear not to wash slides after DRAQ5 staining or colouring will be lost.

Name: Sarah Pringle

Organisation: UMCG 

.........................................

Manufacturer's response: Dear Sarah, Thank you for your helpful reminder. We do indeed emphasise on our official technical datasheet that one should use DRAQ5 last and refrain from washing slides thereafter; if necessary any excess liquid can be tamped away. Kind regards, Roy Edward Sales & Marketing Director

"Great and reliable results"

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining in fixed cell

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is very easy to use. The results are very good and reliable in PFA and methanol fixed cell,. The fluorescence signal is of high qualitiy.

Name: Sabrina Ernst

Organisation: Institute of Biochemistry and Molecularbiology

"It gives us great results, thanks."

PRODUCT:                  DRAQ5

Application Area:        Analyze reactive oxygen species in cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product is easy to use and well-fit for your purpose.

Name: Seung Hyo Lee

Organisation: KAIST 

"Great and dependable nuclear dye"

PRODUCT:                  DRAQ5

Application Area:        Immunofluoresence

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product is very easy to use. Gives great reproducible nuclear staining. We have used this product both in immunofluorescence as a subcellular localisation marker and as a reference dye for plate based assays using a fluorescent reader (In-cell ELISA, ...).

Name: Luc Bertrand

Organisation: University of Miami School of Medicine

"Reliable and sensitive DNA stain"

PRODUCT:                  DRAQ5

Application Area:        immunofluoresence high throughput image analysis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: We found this product reliable and very easy to use. It worked consistently and we were very happy with its application. The product is quite expensive but we found we were able to use it at a lower concentration than recommended with just as great results.

Name: Hayley Newton

Organisation: University of Melbourne

"A very useful product for live cell imaging."

PRODUCT:                  DRAQ5

Application Area:        Microscopy

Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐

Value for Money:        ⭐⭐⭐
Average rating:           2.3

Comments: The dye gives strong staining and could we washed away even from live cells. Nuclei of some cell lines e.g. PC3 could not be stained (the dye does not enter the cells) others e.g H1299 contain strange dotty staining in the cytosol in addition to the nuclear staining. Too high concentration causes artificial condensation of chromatin.

Name: Rastislav Dzijak

Organisation: Institute of organic chemistry and biochemistry

"Works well for cell proliferation assays"

PRODUCT:                  DRAQ5

Application Area:        Quantify cell numbers in response to small molecules in a high-throughput format

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: This product allowed us to perform screens of compounds on cells that could not be assayed using traditional cell proliferation assays like Alamar Blue or MTT. The dye allows rapid quantification of cell numbers in a 96-well plate format. Assay of wells with multiple cell doses generated nice curves that gave us confidence in the quantitative ability of the assay over a wide dynamic range of cell numbers.

Name: Laurie Ailles

Organisation: University Health Network

"Very useful! Using it a lot."

PRODUCT:                  DRAQ5

Application Area:        DNA staining for high-throughput imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Great staining and consistent results. Have used DRAQ5 for the past 5 years.

Name: Diana Stavreva

Organisation: NCI

"Easy to use visualization of DNA"

PRODUCT:                  DRAQ5

Application Area:        stain DNA in living cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Easy to use, good brightness with limited photobleaching and phototoxicity

Name: Claire Walczak

Organisation: Indiana University

"Great and reliable results. Highly recommended."

PRODUCT:                  DRAQ5

Application Area:        To demonstrate binding of extracellular vesicles to leukocytes

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ-5 is easy to use and highly reproducible. it is a go-to reagent for nuclear identification.

Name: Joel Pachter

Organisation: UConn Health

"very useful chemical"

PRODUCT:                  DRAQ5

Application Area:        Analyze cell cycle and otehr parameters in cultured cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Excellent results with this consumable, we use it routinely

Name: Jyotsna Dhawan

Organisation: Institute for Stem Cell Biology and Regenerative Medicine 

"Great reagent, definitely worthy to be purchased and used!"

PRODUCT:                  DRAQ5

Application Area:        Confocal microscopy on Breast Tumor and Lung metastases sections

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a valuable alternative to DAPI if you want to use a red fluorescent dye for staining your nuclei. Its  low-photobleaching, easy to use and yields a low background noise.

Name: Cristina Andreani

Organisation: University of Camerino, MC, Italy (Now @ UTSouthwestern Medical Center, Dallas, Texas) 

"Good staining and good tools for IHF/IF"

PRODUCT:                  DRAQ5

Application Area:        Visualize nuclei in cells and tissus samples

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           3.7

Comments: DRAQ5 is easy to use and fast (30min of incubation) and it stains well.

Name: chloé terciolo

Organisation: INSERM

"Great results"

PRODUCT:                  DRAQ5

Application Area:        Analysing DNA in trypanosoma brucei

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: It is a product, which is easy to use and gives a strong signal.  We used it to stain the nucleus in T.brucei and it is compatible with the oregon green antibody for STED.

Name: Anneliese Hoffmann

Organisation: University of Bern

"Must have tracer "

PRODUCT:                  DRAQ5

Application Area:        nuclear imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Easy-to-use nuclear marker when other fluorescence emissions than blue (DAPI, Hoechst) are required.

Name: Romain Duval

Organisation: Institute fo Research and Development

"Good reproducible results, more comfortable and suitable than DAPI staining"

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining for indirect immunofluorescence experiments

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 was a very useful and easy to use tool for nuclear staining. In my immunofluorescence experiments I need to stain the nuclear fraction of the cells to have appropriate controls and DRAQ5 gave me this opportunity. Moreover, since the detection is at 633 nm laser range, I can use it instead of DAPI and use 405 nm channel for the detection of other dyes, such as Alexa Fluor 405

Name: Antons Martincuks

Organisation: RWTH Aachen University

"Easy to use, reliable, gives consistent results."

PRODUCT:                  DRAQ5

Application Area:        To stain nuclei in fixed cell culture samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It is very easy to use, gives reproducible results, excellent product!  Good value for the price too. Thank you!

Name: Ibolya Andras

Organisation: University of Miami

"GREAT RESULTS"

PRODUCT:                  DRAQ5

Application Area:        LIVE CELL IMAGING MICROSCOPY

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Very easy use and great results for cell live imaging microscopy

Name: José A Sánchez Alcázar

Organisation: Universidad Pablo de Olavide

"Very useful tool, easy to use and it guarantees good results!"

PRODUCT:                  DRAQ5

Application Area:        Analyze the cytotoxicity of compounds in live cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: It is a very easy to use product. This provides high quality results and images working with live cells.

Name: Ananda Rodilla

Organisation: University of Barcelona

"Great product."

PRODUCT:                  DRAQ5

Application Area:        Identification of nucleated cells by flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: Chose DRAQ5 because of its far red emission and near compensation free properties on the system I was using.  The ability to get total nucleated for gating in log space and then DNA index with careful use in a slightly different red channel using linear is a nice plus.

Name: Tony Ward

Organisation: GPB Scientific

"extremely effective and easy to use as Drop & Go"

PRODUCT:                 DRAQ7 DROP & GO
Application Area:        Cell sorting and Cell viability assays

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 For many years, my core facility users in general and I, personally, have been using several BioStatus products, mainly DRAQ5 and DRAQ7. I have always found them effective and extremely easy to use. DRAQ7 has shown for many years to be a fantastic option for the detection and exclusion of dead cells. Quite recently, we have tested and used DRAQ7 DROP & GO for the detection and exclusion of dead cells from a very heterogeneous sample for isolating photoreceptors from adult zebrafish retinae for downstream RT-PCR. It was as easy as described in the name of the product "drop and go". Dead cells were clearly differentiated from the alive ones as can be easily observed in our recently published paper Glaviano et al. "A method for isolation of cone photoreceptors from adult zebrafish retinae" BMC Neuroscience (2016) 17: 71 (DOI 10.1186/s12868-016-0307-2)

Name: Alfonso Blanco

Organisation: University College Dublin

"Very good and reliable reagent."

PRODUCT:                 DRAQ5

Application Area:        Immunocytochemistry, immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to use and cost effective. Great alternative to traditional DAPI in immunocyto-and histochemistry.

Name: Yulia Kiyan

Organisation: Hannover Medical School

"Best nucleic acid stain"

PRODUCT:                  DRAQ5

Application Area:        Staining nuclei in 3D tissue constructs (tissue engineering application)

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Product (DRAQ5) nucleic acid stain works exceptionally well, and keeps common microscope filter channels open since it's a far red stain. Little to no background, easy to use and store. Penetrates deep into thick tissues so you don't observe staining concentration gradients as with other nuclear stains. Perfect for use when imaging 3D tissue constructs with confocal - but also works well for 2D monolayer applications where UV light isn't an option (ex: PMMA microfluidic devices which block UV).

Name: Darren Rodenhizer

Organisation: University of Toronto

"Very useful for in vivo staining, easy to handle, high-quality"

PRODUCT:                  DRAQ5

Application Area:        Staining living mouse embryonic fibroblast nuclei

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: If you are looking for a dye for in vivo staining, DRAQ5 is the best. Even with very little amount of a stock of the product, like 1ul, you can observe bright nuclei immediately. Staining genomic DNA specifically you could capture where DNAs are distributed in nuclei. After decorating your nuclei with it, cells are still happy living healthy. I will keep using the product for my future projects.

Name: Wonki Cho

Organisation: MIT

"Perfect for us."

PRODUCT:                  DRAQ5

Application Area:        Immunohistochemistry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: Easy substitute for DAPI (we had no DAPI laser available).

Name: Elisabeth Naschberger

Organisation: University Medical Center Erlangen

"Great results, easy to apply."

PRODUCT:                  DRAQ5

Application Area:        Analyze toxicity, analyze the process of apoptosis

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: High quality, easy to use, without non-specific staining, good efficiency.

Name: Alicja Warowicka

Organisation: Adam Mickiewicz University, NanoBioMedical Centre

"DRAQ5 is the perfect nuclear stain if no UV-laser is available"

PRODUCT:                  DRAQ5

Application Area:        Nucleus staining in confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: I like that we can stain and visualize nuclei without the need for a UV-laser. The use is very simple, and the sensitivity is very good. We can also differentiate between DRAQ5 and far red probes so we can image several probes in parallel. I also like the fact that the company cares about publications with their products.

Name: Olivia Merkel

Organisation: Wayne State University

"Good Product. Easy to use."

PRODUCT:                  DRAQ5

Application Area:        Staining of Mouse ESC

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Its very easy to use, straight from the fridge without hassle and its reliable.

Name: Hiran Perera

Organisation: UT Health Science Center at Houston 

"We achieved great and specific nuclear staining with this product"

PRODUCT:                  DRAQ5

Application Area:       Immunofluorescence Microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This product is very effective. It is also very potent and has high efficacy when used in amounts under the recommended concentration.  Also, the fact that it can be used in live  cells is a huge benefit.

Name: Courtney Copeland

Organisation: Vanderbilt Uiversity 

"Easy to use DNA stain for debris discrimination."

PRODUCT:                 DRAQ5

Application Area:       Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We use DRAQ5 for debris discrimination in CD34+ cell enumeration tests of blood and harvest samples using lyse no wash method. It works very well combined with CD34 PE and CD45 FITC.

Name: Pal Jakso

Organisation: University of Pecs, Dept. of Pathology  

"Great results"

PRODUCT:                 DRAQ5

Application Area:       Cell culturing for evaluation of biocompatibility

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: Easy to use, fast shipping, reasonable price, good results and good quality

Name: Amirkianoosh Kiani

Organisation: University of New Brunswick

"excellent product, affordable, easy to use and gave great results"

PRODUCT:                 CyGEL
Application Area:        Imaging non-adherent leukemia cell line for biosensor application.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:   ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments:                 We used CyGel for imaging a K562 cell line to verify that our fluorophore-tagged biosensor is internalized by the cells. The product was very easy to use and worked according to the manufacturer’s description. There was no problem with CyGel interfering with our fluorescent signal using a standard fluorescent microscope for cell imaging. We think it was a very reasonable price for the ease of use and results we obtained. While we did not require follow up service, it was there for our assistance as needed.

Name: Steven Ouellette

Organisation: KinaSense LLC

"DRAQ7 is an easy to use viability dye that allows monitoring of cell death over time."

PRODUCT:                 DRAQ7

Application Area:       Cell viability

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ7 was used to measure cell death in complement-dependent-cytotoxicity experiment.  

Our project was to determine the ability of anti-Tn antibody to mediate death of Tn expressing in association with complement. Briefly, Tn expressing cells were incubated with anti-Tn antibody, in the presence of complement and DRAQ7 as a viability marker. Cell were incubated in Incucyte System (www.essenbioscience.com/en/products/incucyte?gclid=CObfsKOZjM8CFWMW0wodVw0JSQ) and the appearance of red fluorescence (staining of dead cells by DRAQ7) was monitored every 30 minutes.  We were able to measure cell death over time by the use of DRAQ7. Fluorescence emission signal was high, and the results obtained were reproducible.

Among all viability dye we have tested, it was the only one that emits strong signal after several hours of incubation.

Name: Daphné Laubreton

Organisation: INRA

"Overall very satisfied with the product and will recommend to all users"

PRODUCT:                 DRAQ5

Application Area:        Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product works great for our applications such as confocal microscopy. We rate this product the best among all the manufacturers. Overall we will order and use this product as long as the prices are kept competitive or we have more superior advantage.

Name: Arun Iyer

Organisation: Wayne State University

"Good product."

PRODUCT:                 DRAQ5

Application Area:        Analyze CTC from blood.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Easy to stain. It was used for stain the nucleus of cells for confocal microscopy.

Name: Xueqing Xia

Organisation: UT MD Anderson Cancer Center