"This product is reliable for efficient staining of the nucleus for live-cell imaging."

 PRODUCT:                 DRAQ5

Application Area:       Live cell imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.7

Comments: It was easy and effective to stain the nucleus. Staining protocol was simple to integrate into the work flow.

Name: JI-YOUNG YOUN

Organisation: LTRI

"Works great"

 PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
 Average rating:          4.0

Comments: Works great. Perfect for confocal. Would recommend.

Name: Mikhail Kolonin

Organisation: UT Houston

"Good value, great results, zero problems with the application."

PRODUCT:                 DRAQ5

Application Area:       localization of cell nuclei

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0

Comments: We are using DRAQ5 for many years to label the cell nuclei in in vitro cultured cells and also in tissue sections. We did not experience any problems. Staining is consistent and easy to detect using confocal microscopy.

Name: Maria Ciemerych

Organisation: University of Warsaw 

"I usually have really great images using Draq5"

PRODUCT:                 DRAQ5

Application Area:       Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0

Comments: Draq5 is extremely easy to use and gives immense signal with absolutely no background. It's a very powerful and useful dye that can be used to study cell nuclei morphology and identifying cell types.

Name: Bernardo Franklin

Organisation: Institute of Innate Immunity  

"Works great! Results obtained with this product were used directly in a publication."

PRODUCT:                 DRAQ5

Application Area:       Applied on thick live midgestation mouse embryo sections.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          4.7

Comments: After an initial titration for our specific tissue/cells, the DRAQ5 dye worked well and provided us with reproducible results. For our tissue (mouse embryo) we ended up using DRAQ5 at a 1:2000 dilution (1:500 proved to be too concentrated).

Name: C Vink

Organisation: The University of Edinburgh  

"DRAQ5 is a very good tool to stain nucleus in live cells"

PRODUCT:                 DRAQ5

Application Area:       nucleus staining in live and fixed cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
 Average rating:          4.7

Comments: In PFA fixed cells DRAQ5 doesn’t gives always the same intensity; it works better in live cells. The staining in live cells is very fast and the nucleus appears very clear and bright.

Name: Silvia Perconti

Organisation: University G d'Annuzuio, Chieti

.........................................

Manufacturer's response: Dear Silvia, thank you for the very constructive comments. We have developed DRAQFx as a standardized reagent specifically for the far-red nuclear counterstaining of fixed cells, as a ready-to-use dropper bottle formulation

"Easy to use and great results."

PRODUCT:                 DRAQ5

Application Area:       Analysis of virus attachment on tissue culture cells.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
 Average rating:          5.0

Comments: We use DRAQ5 to stain tissue culture cells for microscopy studies.  The stain is easy to use and gives a great signal. The results are reproducible.

Name: Anthony Snyder

Organisation: Indiana University

"A really great product, excellent value for money"

PRODUCT:                 DRAQ5

Application Area:       Analysis of cell culture status.

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The product DRAQ5 is easy to use and gives excellent results in confocal microscopy. The protocols for multiple labelling are straightforward and can be applied to a variety of tissues/cells fixed in different way. In the lab, we used it on both tissues (normal or tumors) and cell cultures (cell lines and primary culture), always with success. A plus is the fact that the staining does not interfere with other staining, nor does it bleach other fluorophores.

Name: Carla Mucignat

Organisation: University of Padova (Italy)

"Great results."

PRODUCT:                 DRAQ5

Application Area:       Immunofluorescence

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: DRAQ5 is very user friendly. We used it at 2.5 microM concentration to visualize nuclear/mitotic DNA of U2OS cells. Incubation of DRAQ5 was done during the penultimate wash in our immunostaining protocol (incubation ~ 5min). Following a final wash in PBS, the slides were mounted and images acquired on an LSM510 confocal microscope.

Name: Goedele Maertens

Organisation: Imperial College London

"Nice staining with no dirty background; very good results."

PRODUCT:                 DRAQ5

Application Area:       DNA staining on retinal cell after transplantation

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: very easy to use. very good reproducibility in different experiments. very cheap product.

Name: Olivier Goureau

Organisation: INSERM, France

"Great results"

PRODUCT:                 DRAQ5

Application Area:       Protein translocation using image stream

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: The nucleus staining is very easy to use. It was stained 1:1000 in PBS Buffer and analysed by Amnis image stream

Name: Juliane Klein

Organisation: Institut für Immunolgie

"Absolutely necessary to document movement of iron through intestinal cells"

PRODUCT:                 DRAQ5

Application Area:       Analysis of metabolites within cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Extremely easy to use.  Highly reproducible results.  Easy to quantify.

Name: Jonathan Glass

Organisation: Feist-Weiller Cancer Center, LSU Health Sciences Center

"Excellent DNA dye - we use for single staining in antiparasitic high content assays"

PRODUCT:                 DRAQ5

Application Area:       Fluorescence microscopy in high content screening

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: Ideal for single-staining high content assays, DRAQ5 enables clear visualization of cell nucleus and gives a  smoother background signal at cell cytoplasm - which is enough for image processing softwares to delimitate and segment cellular structures properly. Moreover, the stain stability enables material storage for longer period than other conventional dyes (we have imaged 384-well plates after 4 weeks post-staining and the fluorescence signal was sufficiently high); also there is no need for dye washout after staining the microplates (less pipetting stesp). We recommend DRAQ5 in single-staning high content screening for assessing compound activity against intracellular trypanosomatids (Trypanosoma cruzi and Leishmania spp.) - the dye marks both DNA material from intracellular parasites and host cells - and providing a faint signal in the cytoplasm - turning it easy to segment and quantify the intracellular parasites.

Name: Caio Haddad Franco

Organisation: Instituto Butantan

"Excellent cell imaging pictures"

PRODUCT:                 DRAQ5

Application Area:       Checking interactions between cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: This dye helps us to check synapsis between mast cells and pancreatic cancer cells. These findings provide evidence for a novel mode of unidirectional crosstalk between MCs and cancer cells implicating direct activation by cancer cells in MC reprogramming into a pro tumorigenic profile

Name: Gorzalczany Yaara

Organisation: Tel Aviv University 

"This is a product I intend to use, for as long as they produce it."

PRODUCT:                 DRAQ5

Application Area:       HIV infection and signaling cascades in cell samples

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a simple, easy-to-use product. Either used on live-primary or fixed cells a simple 5-10 minute incubation followed by washing steps produced consistent, repetitive results. Its fluorescent excitation/emission spectra are very well suited to combine with other fluorophores without channel bleeding or crosstalk. The particular spectral characteristics of DRAQ5 make it also easy to work with in fluorospectrometry-based experiments. We have  also used DRAQ5 combined with metabolic biomarkers in solution without observing interference with cellular metabolic uptake in short-time uptake experiments, this allowed us not only to reduce experimental time, but also helped us reduce cell manipulation.

Name: VICTOR CASTRO VILLELA

Organisation: UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE

"Easy way to add valuable data to all our experiments"

PRODUCT:                 DRAQ7

Application Area:       Monitor cell viability

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Great value, straightforward way to assess cell viability through flow cytometry in all our experimental settings. Easily incorporated into the work flow to provide robust and easy to interpret data.

Name: Cathy Thornton

Organisation: Swansea University

"Good results and quite easy to use"

PRODUCT:                 DRAQ5

Application Area:       Tissue Engineering

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: For Draq5, it was easy to employ and visualize. As a fluorescent dye, the product was highly specific for DNA and there was no smearing problem

Name: Engin Pazarçeviren

Organisation: Middle East Technical university

"An excellent quality product that we will continue to use"

PRODUCT:                 DRAQ5

Application Area:       Imaging flow cytomtery for DNA repair biomarkers in human cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.3

Comments: The Draq 5 product is of excellent quality and is reliable and reproducible under experimental conditions. In addition it is very easy to use. We have used the product for the majority of our imaging flow cytometry applications and have published extensively on this DNA repair/DNA damage and human genotoxicity. We will continue to use the Draq 5 formulation for our work although the price is rather too high

Name: Christopher Parris

Organisation: Anglia Ruskin University, Cambridge, UK

"Very bright photostable far red dye"

PRODUCT:                 DRAQ7

Application Area:       Flow cytometry

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: The dye was easily loaded into cells at 37C giving a high level of fluorescence at 760 nm making it easy to gate out dead cells on flow cytometric dot-plots. There was no apparent photo-bleaching of this far red dye either, quite impressive.

Name: Gary Warnes

Organisation: Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University of London, UK

"Great results"

PRODUCT:                 DRAQ5

Application Area:       Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Excellent product with reproducible data. I would recommend to use other researcher

Name: Yogesh Singh

Organisation: Tübingen University, Tübingen, Germany

"Overall the product was effective."

PRODUCT:                  DRAQ5

Application Area:        Analyze acrosome exocytosis in sperm

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: Product was easy to store/handle.  Purchase of product was easy. Preparation of the product took more time than expected but was due to the experimental protocol and not the product itself. We would purchase this product again for further research if needed.

Name: Melissa Pecoraro

Organisation: Androvia LifeSciences

"Great result for publication."

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: It works very well and specifically. It is easy to use.

Name: Bin Wang

Organisation: Thomas Jefferson University

"Easy to use reagent. Really precise and perfect for in-cell western experiments!"

PRODUCT:                  DRAQ5

Application Area:        Normalisation of cell number during in-cell western blot experiments

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: I used DRAQ5 for internal normalisation of in-cell western (ICW) experiments to detect cytosine modifications in human cardiac mesenchymal cells. It arrived few days after my order. It is really easy and fast to use: it can be added at the end of the standard ICW procedure  before plate reading. Really accurate and reproducible.

Name: Francesco Spallotta

Organisation: Goethe University - Frankfurt am Main (DE)

"Quality product, good value for money"

PRODUCT:                  DRAQ7

Application Area:        Immunometabolism

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: Very easy to use, gives clear, consistent and reproducible results, which makes measuring cell death easy and reliable. Would strongly recommend this product for measuring apoptosis. Good value for money.

Name: Nigel Francis

Organisation: Swansea University

"Nice reagent for cell cycle analysis"

PRODUCT:                  DRAQ5

Application Area:        Analysis of cell cycle status in colorectal cancer cells

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is a useful reagent for analysing the cell cycle status of live/dead cells by flow cytometry. For our purposes, cells were first killed by fixation in 70% Ethanol  (because we were co-staining for other antigens) and eventually resuspended in buffer containing 2ul/ml DRAQ5 allowing 10 mins prior to flow cytometric analysis. Lovely DNA profiles were obtained with no compensation issues with the other fluorochrome (PE) used in the study. Never attempted on live cells but we understand from other colleagues in the department that this is possible. Care must be taken when handling as this is a toxic substance.

Name: Rhys Morgan

Organisation: University of Bristol

"Essential dye for my laboratory"

PRODUCT:                  DRAQ5

Application Area:        Live staining of nuclei for cell imaging

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: DRAQ5 is consistently easy to use and produces excellent staining of live cell nuclei, for living cell imaging.

Name: Jennifer G.

Organisation: CFRC

"Great result"

PRODUCT:                  DRAQ7

Application Area:        Cell Biology

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: Good and useful product to stain nuclei in our experiments

Name: Serge Benichou

Organisation: Institut Cochin

"Easy to analyze apoptosis in cells at certain phases of the cell cycle"

PRODUCT:                  DRAQ5

Application Area:        Analyze apoptosis in cells at certain phases of the cell cycle

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product is very easy to use for cytometry and results are reproducible. We perform simultaneous staining with Draq5 and Annexin-V on multiple myeloma cell line exposed to drugs and we follow their apoptosis at certain phases of the cell cycle.

Name: Susana Hernandez garcia

Organisation: Cancer Research Institute of Salamanca CSIC-USAL University

"Great product, will use it again"

PRODUCT:                  DRAQ7

Application Area:        Analyse impact of components on the fraction of compromised cells in drug delivery / drug uptake stu

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product is easy to use, creates reliable results when comparing to other complementary techniques.

Name: Hanne Nielsen

Organisation: University

"Good nuclear stain, very easy to use."

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining, used together with polyclonal Ab and phalloidin, for confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           4.0

Comments: Effective and easy to use, the signal could be weak sometimes, but this could have been experimental variation or the microscope itself. Had no problem being able to pseudo-colour and the images and contrast with other stains was generally very good. The sales/technical team were very good before purchase, after-sales care not really applicable, as we didn't need to get in touch after buying it.

Name: Elisabeth Dyrynda

Organisation: Heriot Watt University

"Good nuclear stain that fluoresces in the far red."

PRODUCT:                  DRAQ5

Application Area:        High content imaging.

Ease of Use:              ⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.3

Comments: DRAQ5 is great as it can be used to stain living cells as well as fixed ones.  Of course, the cells won't  divide in it, so you can't use it for long-term time-lapse imaging, but it's still very useful for shorter time lapses.

Name: Bilal Zulfiqar

Organisation: Griffith University

.........................................

Manufacturer's response: Dear Bilal, thanks for your helpful feedback on DRAQ5. This is important information – we do not recommend DRAQ5 for long-term exposure to live cells as it, like ALL high affinity DNA binders and intercalators, will have some kind of impact on cell health. There are exceptions especially with terminally-differentiated cells as observed with HUVEC, macrophages and BaF3. However, short-term exposure and use in live-endpoint assays has been a hallmark of the product’s success in drug discovery and flow cytometry applications, in particular the loss of cytoplasmic GFP signals when cells are partially permeabilized by fixation with formaldehyde and the unspecific loss of cells due to RBC lysis and associated washes, respectively.

"Perfect for our needs, and gave consistent results."

PRODUCT:                  DRAQ5

Application Area:        Nuclear staining of primary fibroblasts

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.7

Comments: Product was straight forward to use, fast and consistent. Perfect for the application we required as a counter-stain for immunofluorescence using green and red fluorophores.  Product lasted a long time (if stored correctly) and gave robust results until the last drop. The instructions which came with the product were straight forward and easy to follow for consistent results.

Name: Keith Wheaton

Organisation: University of Ottawa

"Great results"

PRODUCT:                  DRAQ5

Application Area:        Implant fabrication

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐
Average rating:           3.7

Comments: Good product/ fast delivery/ in general i do recommend it to people in the field

Name: A Kiani

Organisation: UNB

''I loved the images obtained from the DRAQ5"

PRODUCT:                  DRAQ5

Application Area:        in vitro aplications

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We had very nice results using DRAQ 5 and we have published recently a paper. The price was a bit high.

Name: Diaconeasa Maria

Organisation: Universitatea de Ştiinţe Agricole şi Medicină Vete

"Easy to use dye for analysis of DNA content without compensation issues in flow."

PRODUCT:                  DRAQ5

Application Area:        immunology, B-cell development and leukemia

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.0

Comments: Easy to use, good staining, no compensation issues in flow cytometry.

Name: Vincent van der Velden

Organisation: Erasmus MC, Immunology

"Great product! It is absolutely vital for our lab, no nice photos without DRAQ5!"

PRODUCT:                  DRAQ5

Application Area:        Analyze cell culture metabolites - confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐
Average rating:           4.3

Comments: The product is very easy to use for microscopy, in few minutes your sample is colored.

I will recommend to researchers to use it if you have laser or filters for it.

It is reliable and results are reproducible.

We perform studies on melanoma and retina cell culture, exposed to different stress conditions and we follow their morphology after treatment.

Name: Dumitrita Rugina

Organisation: University of Agricultural Sciences and Veterinary Medicine

"great images and can use in far red region along with green fluorescent fluorophores"

PRODUCT:                  DRAQ5

Application Area:        To view the nuclei for imaging in confocal microscopy in the far-red region

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.7

Comments: easy to use and high reproducibility and can image immediately.

Name: Anita chalasani

Organisation: Wayne State University

"Great results"

PRODUCT:                  DRAQ5

Application Area:        Confocal microscopy

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.3

Comments: DRAQ5 is a good staining to see nucleus in liver cells and tissues. It is efficient in both  paraffin-embedded and OCT-frozen tissue sections. It is easy to use and the results are excellent.

Name: Anna Alisi

Organisation: Bambino Gesù Children's Hospital and Research Institute

"An excellent product"

PRODUCT:                  DRAQ5

Application Area:        Visualize nuclei using confocal microscopy

Ease of Use:              ⭐⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           5.0

Comments: We used DRAQ5 to label the nuclei of live adult rat ventricular myocytes (see Weeks et. al. β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. Journal of the American Heart Association. 2017 6(4)). We labelled the cells with 1 uM DRAQ5 for 10 minutes prior to imaging, which was lower than the recommended concentration, but it fluoresced nicely.

Name: Kate Weeks

Organisation: Baker Heart and Diabetes Institute

"Great results"

PRODUCT:                  DRAQ5

Application Area:        To analyze nuclei in the cultured isolated bovine chondrocytes

Ease of Use:              ⭐⭐⭐⭐
After Sales Service:    ⭐⭐⭐

Value for Money:        ⭐⭐⭐⭐⭐
Average rating:           4.0

Comments: The dye was highly effective as even at very diluted concentrations produced excellent results. The confocal images obtained from these chondrocytes whose nuclei were labelled with DRAQ5 produced high quality and reproducible results. I had used this dye to count the number of cells in clusters because in my culture experiments the cell membranes were frequently touching and therefore thresholding of individual cells was impossible. In order to count number of cells in clusters I counted number of nuclei and DRAQ5 gave me highly reliable results as I could count the number of nuclei in 3D by using Volocity (a 3D image analysis software). It is quite an expensive dye but as the very diluted concentrations are very effective therefore, justifies the cost. Moreover, it was easy to use and highly penetrable and required very little time to label nuclei efficiently.

Name: Asima Karim

Organisation: The University of Edinburgh